| Project/Area Number |
24880005
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHIMURA Hanako 北海道大学, (連合)農学研究科(研究院), 助教 (20507230)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ウイルス / サテライトRNA / RNAサイレンシング / 病徴誘導 |
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| Research Abstract |
Cucumber mosaic virus (CMV) Y satellite RNA (Y-sat) specifically induces a bright yellow mosaic on tobacco through RNA silencing machinery. In this study, I investigated the roles of major RNA silencing components such as DCLs and RDR6 in the Y-sat-mediated yellow induction. By using a virus-induced gene silencing strategy against DCLs and RDR, I found that the accumulation levels of CMV RNAs, Y-sat, DCL2, DCL4 and RDR6 mRNAs changed in a complicated manner interfering with each other. Based on transcript analyses, it was found that DCL4 was more important than DCL2 in affecting the accumulation levels of CMV and Y-sat and thus to generate 21-nt siRNAs of Y-sat. It was also found that Y-sat siRNA levels were positively correlated with the degree of yellowing. In addition, the yellow induction by Y-sat was apparently independent of RDR6, but RDR6 indirectly affects the yellow symptoms because decreased levels of RDR6 enhanced Y-sat siRNA levels and eventually enhanced yellowing.
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