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Update of breed improvement system using grafting and phloem transportable RNA.

Research Project

Project/Area Number 24880007
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionHirosaki University

Principal Investigator

KASAI Atsushi  弘前大学, 農学生命科学部, 研究員 (80633982)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物育種学 / NBT / 接ぎ木 / RNAサイレンシング / RdDM / エピゲノム編集 / アグロインフィルトレーション / 篩管長距離輸送サイレンシングシグナル / TGS / GrIGS / 輸送モチーフ
Research Abstract

GrIGS (Graft induced gene silencing) system is the epigenome editing induction method to a graft partner via long-distance transportable promoter-targeting siRNA through the phloem. The application of this system is one of the new plant breeding techniques (NBT) to improve the cultivars of various horticultural crops. Since the produced plants do not contain any transgenes, these do not correspond to GM plants. In this study, I examined the improvement of this system and its application. As a result, epigenome editing was induced efficiently by including transient expression assay of the transportable signals and signal amplification system, furthermore also in the endogenous gene promoter of tomato.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (17 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (11 results) (of which Invited: 1 results)

  • [Journal Article] 植物におけるエピゲノム編集-その原理と接ぎ木による誘導2014

    • Author(s)
      葛西厚史、原田竹雄
    • Journal Title

      生物の科学遺伝

      Volume: 68(2) Pages: 140-144

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 植物におけるエピゲノム編集―その原理と接ぎ木による誘導2014

    • Author(s)
      葛西厚史・原田竹雄
    • Journal Title

      生物の科学 遺伝

      Volume: 68(2) Pages: 140-144

    • Related Report
      2013 Annual Research Report
  • [Journal Article] エピ変異誘導による品種改良 ~遺伝子の環境記憶システムの活用~2014

    • Author(s)
      原田竹雄・葛西厚史
    • Journal Title

      果実日本

      Volume: 69(2) Pages: 90-93

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Scion on a stock producing siRNAs of potato spindle tuber viroid (PSTVd) attenuates accumulation of the viroid2013

    • Author(s)
      Kasai A, Sano T, Harada T
    • Journal Title

      PLOS ONE

      Volume: 8(2) Issue: 2 Pages: e57736-e57736

    • DOI

      10.1371/journal.pone.0057736

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の接ぎ木を利用した接ぎ木相手の形質転換2013

    • Author(s)
      葛西厚史・原田竹雄
    • Journal Title

      植物の成長調節

      Volume: 48(2) Pages: 125-134

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 植物育種技術としてのエピ変異体作出法;シリーズ:NBT⑤2013

    • Author(s)
      葛西厚史・原田竹雄
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 Pages: 462-465

    • Related Report
      2013 Annual Research Report
  • [Presentation] ジャガイモの新奇エピゲノム編集法2014

    • Author(s)
      葛西厚史・北條初音・原田竹雄
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] An effective TGS induction in grafting partner by phloem transport of siRNA and mRNA2013

    • Author(s)
      Kasai A、Iwashiro R、Harada T
    • Organizer
      Plant Biology 2013
    • Related Report
      2013 Final Research Report
  • [Presentation] An effective TGS induction in grafting partner by phloem transport of siRNA and mRNA2013

    • Author(s)
      Atsushi Kasai, R Iwashiro, T Harada
    • Organizer
      Plant Biology 2013
    • Place of Presentation
      PROVIDENCE, RHODE ISLAND
    • Related Report
      2013 Annual Research Report
  • [Presentation] Epimutant generation of tomato SlVirp1 and its characteristics2013

    • Author(s)
      Hatsune Hojo, A Kasai, T Harada
    • Organizer
      Plant Biology 2013
    • Place of Presentation
      PROVIDENCE, RHODE ISLAND
    • Related Report
      2013 Annual Research Report
  • [Presentation] 接ぎ木を利用したエピ変異体獲得について2012

    • Author(s)
      葛西厚史、北條初音、川又奨、原田竹雄
    • Organizer
      第35回日本分子生物学会年会
    • Year and Date
      2012-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 低アミロースを生産するジャガイモエピ変異体の作出

    • Author(s)
      辻村玲和・北條初音・葛西厚史・原田竹雄
    • Organizer
      第8回東北育種研究集会
    • Place of Presentation
      弘前大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Epimutant induction by long-distance transport siRNA

    • Author(s)
      Atsushi Kasai, H Hojo, T Harada
    • Organizer
      日本植物細胞分子生物学会 8th Joint Korea-Japan Plant Biotech Workshop -New Biotechnology-based Plant Breeding Techniques-
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ウイロイド(PSTVd)のsiRNAs産生台木上穂木のPSTVd増殖に対する抵抗性

    • Author(s)
      葛西厚史ら
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] エピ変異体獲得におけるRNA篩管輸送モチーフの活用

    • Author(s)
      原田竹雄ら 葛西厚史
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 接ぎ木を利用したエピ変異体獲得について

    • Author(s)
      葛西厚史ら
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] トマトSlVirp1エピ変異体の獲得とその特性

    • Author(s)
      葛西厚史ら
    • Organizer
      日本育種学会第123回講演会
    • Place of Presentation
      東京農業大学
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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