| Project/Area Number |
24880011
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOSHIDA Ayako 東京大学, 生物生産工学研究センター, 特任助教 (90633686)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | メカノセンシティブチャネル / グルタミン酸発酵 / コリネバクテリウム グルタミカム / 膜タンパク質 / X線結晶構造解析 / グルタミン酸醗酵 / コリネバクテリウム / 応用微生物学 / アミノ酸発酵 / タンパク質X線結晶構造解析 |
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| Research Abstract |
Corynebacterium glutamicum is a bacterium for glutamate fermentation. Recently, the mechanosensitive channel, NCgl1221 is uncovered to be responsible for the export of glutamate and is important for the glutamate fermentation. To elucidate the mechanism of glutamate exporting system in C. glutamicum structurally, we performed the crystallographic analysis of NCgl1221
We constructed the expression system of NCgl1221 in Escherichia coli and we could successfully obtain purified NCgl1221. After crystallization screening, protein crystals appeared in several conditions. Although the resolution was poore, we could observe the good diffraction patterns from the crystals obtained in the optimized crystallization condition.
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