Identification and functional analysis of small RNAs packaged into influenza virion
Project/Area Number |
24880014
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Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Applied veterinary science
|
Research Institution | The University of Tokyo |
Principal Investigator |
MURAKAMI Shin 東京大学, 農学生命科学研究科, 特任助教 (10636757)
|
Project Period (FY) |
2012-08-31 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | インフルエンザ / ウイルス粒子 / non-coding RNA |
Outline of Final Research Achievements |
It is not known whether the various small RNAs, such as small RNA derived from host cells are incorporated in influenza A virus particles. Small RNAs which were extracted from viral particles grown in embryonated chicken eggs or in Vero cells were analyzed using the next generation sequencers. As a result, host-derived tRNA and viral genomic RNA fragments were detected. We established a MDCK cells stably expressing the RNA fragment of HA segment (MDCK-5’ HA) and examined viral growth in the cells. Viral growth kinetics in MDCK-5’ HA were similar to those in wild-type MDCK cells. The expression level and localization in virus-infected cells may differ from that in the MDCK-5’ HA cells. It is necessary to prepare a cells expressing amount increased viral genome fragments and to analyze in such cells.
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Report
(3 results)
Research Products
(3 results)