| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
It is not known whether the various small RNAs, such as small RNA derived from host cells are incorporated in influenza A virus particles. Small RNAs which were extracted from viral particles grown in embryonated chicken eggs or in Vero cells were analyzed using the next generation sequencers. As a result, host-derived tRNA and viral genomic RNA fragments were detected. We established a MDCK cells stably expressing the RNA fragment of HA segment (MDCK-5’ HA) and examined viral growth in the cells. Viral growth kinetics in MDCK-5’ HA were similar to those in wild-type MDCK cells. The expression level and localization in virus-infected cells may differ from that in the MDCK-5’ HA cells. It is necessary to prepare a cells expressing amount increased viral genome fragments and to analyze in such cells.
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