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Malate dynamics in symbiosis between Lotus japonicus and Mesorhizobium loti

Research Project

Project/Area Number 24880020
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionKyoto University

Principal Investigator

TAKANASHI Kojiro  京都大学, 生存圏研究所, 助教 (10632119)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsミヤコグサ / リンゴ酸 / ALMT / SWEET / 共生窒素固定 / 根粒菌
Research Abstract

Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrate, and also various minerals, the knowledge on molecular basis of plant transporters that mediate those metabolite exchanges are still limited. In this study, I characterized two transporter families, ALMT and SWEET. One of LjALMT gene and LjSWEET gene express in nodule vascule bundles, and their transport activities for malate and sucrose were confirmed with Xenopus oocyte and FLET, respectively. The direct involvement of these transporters in nutrient transport into bacteroids was not observed, however, their functions in carbon dynamics in nodules were suggested.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (34 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (27 results) (of which Invited: 2 results) Book (3 results) Remarks (1 results)

  • [Journal Article] EjMATEI, a citrate transporter responsible for iron supply to nodule infection zone of Lotus japonicus.2013

    • Author(s)
      Takanashi, K., et al.
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 4 Pages: 585-594

    • DOI

      10.1093/pcp/pct019

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis ABCB21 is a facultative auxin im/exporter regulated by cytoplasmic auxin concentration2012

    • Author(s)
      Kamimoto, Y., et al.
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Issue: 12 Pages: 2090-2100

    • DOI

      10.1093/pcp/pcs149

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue-specific transcriptome analysis in nodules of Lotus japonicus2012

    • Author(s)
      Takanashi, K., et al
    • Journal Title

      Mol Plant Microbe Interact.

      Volume: 25(in press) Issue: 7 Pages: 869-876

    • DOI

      10.1094/mpmi-01-12-0011-r

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Functional Aaalysis of LjSWEET3, a candidate of sugar transporter in the nodule of Lotus japonicus2014

    • Author(s)
      Mayuko Yoshimizu, Akifumi Sugiyama, Yuka Saida, Kojiro Takanashi, Davide Sosso, Wolf B Frommer,, Kazufumi Yazaki
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Year and Date
      2014-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of LjSWEET3, a candidate of sugar transporter in the nodule of Lotus japonicus2014

    • Author(s)
      Mayuko Yoshimizu, Akifumi Sugiyama, Yuka Saida, Kojiro Takanashi, Davide Sosso, Wolf B Frommer, Kazufumi Yazaki
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山大学(富山)
    • Related Report
      2013 Annual Research Report
  • [Presentation] LjMATE1 assists the Fe translocation to nodules by providing citrate2013

    • Author(s)
      Kojiro Takanashi, Kengo Yokosho, Hirokazu Takahashi, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Mikio Nakazono, Jian Feng Ma, Kazufumi Yazaki
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎
    • Year and Date
      2013-10-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ミヤコグサ根粒で発現する糖輸送体候補遺伝子LjSWEET3の解析2013

    • Author(s)
      吉水麻祐子、杉山暁史、齊田有桂、高梨功次郎、Davide Sosso、Wolf B Frommer、矢崎一史
    • Organizer
      第31回日本植物細胞分子生物学会
    • Place of Presentation
      札幌
    • Year and Date
      2013-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of MATE - type transporters, LjMATE2 and LjMATE3 in Lotus japonicus2013

    • Author(s)
      Yoshihiro Ota, Kojiro Takanash1, Akifumi Sugiyama, Toshio Aoki, Kazufumi Yazaki
    • Organizer
      International Workshop on Plant Membrane Biology XVI
    • Place of Presentation
      倉敷
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a SWEET transporter in nodules of Lotus japonicus2013

    • Author(s)
      Yuka Saida, Akifumi Sugiyama, Kojiro Takanashi, Davide Sosso, Wolf B Frommer,, Kazufumi Yazaki
    • Organizer
      International Workshop on Plant Membrane Biology XVI
    • Place of Presentation
      倉敷
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Biochemical analysis of flavonoid secretion in soybean2013

    • Author(s)
      Kazuaki Yamashita、Akifumi Sugiyama、Kojiro Takanashi、Kazufumi Yazaki
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of LjSWEET4 in nodules of Lotus japonicus2013

    • Author(s)
      Yuka Saida, Akifumi Sugiyama, Kojiro Takanashi, Kazufumi Yazaki
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ミヤコグサのMATE型輸送体LjMATE2およびLjMATE3の解析2013

    • Author(s)
      太田喜寛、高梨功次郎、杉山暁史、青木俊夫、矢崎一史
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] LjMATE1 assists the Fe translocation to nodules by providing citrate2013

    • Author(s)
      Kojiro Takanashi, Kengo Yokosho, Hirokazu Takahashi, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Mikio Nakazono, Jian Feng Ma, Kazufumi Yazaki
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      Phoenix Seagaia Resort(宮崎)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ミヤコグサ根粒で発現する糖輸送体候補遺伝子LjSWEET3の解析2013

    • Author(s)
      吉水麻祐子、杉山暁史、齊田有桂、高梨功次郎、Davide Sosso、Wolf B Frommer、矢崎 一史
    • Organizer
      第31回日本植物細胞分子生物学会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ミヤコグサ根粒における糖輸送体候補SWEETの解析2012

    • Author(s)
      齊田有桂、杉山暁史、高梨功次郎、矢崎一史
    • Organizer
      分子生物学会2012年度大会
    • Place of Presentation
      博多
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズ根のフラボノイド分泌に関わるダイズ遺伝子の探索2012

    • Author(s)
      ⑩山下和晃、杉山暁史、高梨功次郎、矢崎一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸
    • Year and Date
      2012-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] ミヤコグサ根粒で発現するSWEET輸送体の機能解析2012

    • Author(s)
      ⑪齊田有桂、杉山暁史、高梨功次郎、矢崎一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸
    • Year and Date
      2012-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] ミヤコグサで発現するMATE型輸送体LjMATE2およびLjMATE3の解析2012

    • Author(s)
      ⑫太田喜寛、高梨功次郎、杉山暁史、青木俊夫、矢崎一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸
    • Year and Date
      2012-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] ミヤコグサ根粒で機能する膜輸送体の解析2012

    • Author(s)
      ⑬高梨功次郎
    • Organizer
      第6回ダイズ研究会
    • Place of Presentation
      佐賀
    • Year and Date
      2012-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a SWEET transporter in nodules of Lotus japonicus

    • Author(s)
      Saida Y, Sugiyama A, Takanashi K, Sosso D, Frommer WB, Yazaki K
    • Organizer
      International Workshop on Plant Membrane Biology XVI
    • Place of Presentation
      倉敷市芸文館(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of MATE-type transporters, LjMATE2 and LjMATE3 in Lotus japonicus

    • Author(s)
      Yoshihiro Ota, Kojiro Takanashi, Akifumi Sugiyama, Toshio Aoki, Kazufumi Yazaki
    • Organizer
      International Workshop on Plant Membrane Biology XVI
    • Place of Presentation
      倉敷市芸文館(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ダイズにおけるフラボノイド分泌機構の生化学的解析

    • Author(s)
      山下 和晃、杉山 暁史、高梨 功次郎、矢崎 一史
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(宮城)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサのMATE型輸送体 LjMATE2およびLjMATE3の解析

    • Author(s)
      太田喜寛, 高梨功次郎, 杉山暁史, 青木俊夫, 矢崎一史
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Characterization ofLjSWEET4 in nodules of Lotus japonicus

    • Author(s)
      Yuka Saida,Akifumi Sugiyama,Kojiro Takanashi,KazufumiYazak
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサ根粒における糖輸送体候補SWEETの解析

    • Author(s)
      齊田有桂、杉山暁史、高梨功次郎、矢崎 一史
    • Organizer
      分子生物学会2012年度大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサ根粒で発現するSWEET輸送体の機能解析

    • Author(s)
      齊田有桂、杉山暁史、高梨功次郎、矢崎 一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸大学(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ダイズ根のフラボノイド分泌に関わるダイズ遺伝子の探索

    • Author(s)
      山下 和晃、杉山 暁史、高梨 功次郎、矢崎 一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸大学(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサで発現するMATE 型輸送体LjMATE2 および LjMATE3 の解析

    • Author(s)
      太田喜寛, 高梨功次郎, 杉山暁史, 矢崎一史
    • Organizer
      第22回植物微生物研究交流会
    • Place of Presentation
      神戸大学(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサ根粒で機能する膜輸送体の解析

    • Author(s)
      高梨功次郎
    • Organizer
      第6回ダイズ研究会
    • Place of Presentation
      佐賀大学(佐賀)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ミヤコグサ根粒で機能する膜輸送体

    • Author(s)
      高梨功次郎
    • Organizer
      岡山大学資源植物科学研究所 拠点共同研究主催ワークショップ 『植物ホルモンとイオン輸送体の相互理解』
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] ABC Proteins and Other Transporters in Lotus japonicus and Glycine max, M. Geisler (ed.), Plant ABC Transporters, Signaling and Communication in Plants 22, doi:10.1007/978-3-319-06511-3_102014

    • Author(s)
      Takanashi K. and Yazaki K
    • Related Report
      2013 Final Research Report
  • [Book] ABC proteins and other transporters in Lotus japonicus and Glycine max. In: Geisler, M. and Baluska, F. (eds.) Plant ABC transporters2014

    • Author(s)
      Takanashi, K., Yazaki, K.
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] 「二結晶型分光器を用いた蛍光X線分光法で植物中の元素の振る舞いを調べる」バイオサイエンスとインダストリー(2013)712013

    • Author(s)
      杉山暁史、高梨功次郎、伊藤嘉昭、福島整
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LPGE/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2012-11-27   Modified: 2019-07-29  

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