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Examination of somatic cell reprogramming efficiency with plant gene

Research Project

Project/Area Number 24880023
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionKyoto University

Principal Investigator

SEMI Katsunori  京都大学, iPS細胞研究所, 研究員 (90633058)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords体細胞初期化 / iPS細胞 / DNAメチル化 / 植物遺伝子
Research Abstract

The global epigenetic alteration is induced by the expression of reprogramming factors during the somatic cell reprogramming. To understand of genome-wide epigenetic alteration, it is useful for the elucidation of the reprogramming mechanism. To analyze the genome-wide DNA methylation during the somatic cell reprogramming process, we performed DNA methylation analysis by RRBS methods. This result showed tumor cells gained de novo DNA methylation at ESC-methylated region, whereas kidney-methylated region retained their original methylation status. And these tumor cells have no genetic mutation at oncogenes. These results suggest that incomplete reprogramming can induce the tumor cell by the epigenetic abnormal alteration. Further, we performed forced expression of reprogramming factors with epigenetic modifier which derived from plant gene, but efficiency was not dramatically improved.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (19 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Book (4 results) Remarks (4 results)

  • [Journal Article] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation.2014

    • Author(s)
      Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y.
    • Journal Title

      Cell

      Volume: 156 Issue: 4 Pages: 663-677

    • DOI

      10.1016/j.cell.2014.01.005

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chromatin accessibility at a STAT3 target site is altered prior to astrocyte differentiation2013

    • Author(s)
      Urayama S, Semi K, Sanosaka T, Hori Y, Namihira M, Kohyama J, Takizawa T, Nakashima K
    • Journal Title

      Cell Struct Funct

      Volume: 38(1) Pages: 55-66

    • NAID

      130004053894

    • URL

      https://www.jstage.jst.go.jp/article/csf/38/1/38_12034/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Keratinocyte stem cells but not melanocyte stem cells are the primary target for radiation-induced hair graying.2013

    • Author(s)
      Aoki H
    • Journal Title

      J Invest Dermatol

      Volume: 155 Pages: 600-10

    • DOI

      10.1172/jci63572

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular reprogramming and cancer development.2013

    • Author(s)
      Semi K, et al.
    • Journal Title

      Int J Cancer.

      Volume: 132(6) Issue: 6 Pages: 1240-1248

    • DOI

      10.1002/ijc.27963

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromatin Accessibility at a STAT3 Target Site Is Altered Prior to Astrocyte Differentiation2013

    • Author(s)
      Satoshi Urayama
    • Journal Title

      Cell Structure and Function

      Volume: 38 Issue: 1 Pages: 55-66

    • DOI

      10.1247/csf.12034

    • NAID

      130004053894

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Treatment of a mouse model of spinal cord injury by transplantation of human iPS cell-derived long-term self-renewing neuroepithelial-like stem cells2012

    • Author(s)
      Fujimoto Y., Abematsu M., FalkA., Tsujimura K., Sanosaka T., Juliandi B.. Semi K., Namihira M., Komiya S., SmithA., Nakashima K,
    • Journal Title

      Stem Cells

      Volume: 30 Issue: 6 Pages: 1163-1173

    • DOI

      10.1002/stem.1083

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation2014

    • Author(s)
      Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y
    • Organizer
      ISSCR
    • Place of Presentation
      Canada, Vancouver
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of genomic imprinting during iPS cell derivation2014

    • Author(s)
      八木正樹,蝉克憲, Woltjen Knut,山中伸弥,山田泰広
    • Organizer
      日本発生生物学会大会
    • Place of Presentation
      愛知県
    • Related Report
      2013 Final Research Report
  • [Presentation] PREMATURE TERMINATION OF REPROGRAMMING IN VIVO LEADS TO CANCER DEVELOPMENT THROUGH ALTERED EPIGENETIC REGULATION.2014

    • Author(s)
      Semi K, Ohnishi K, Yamamoto T, Tanaka A, Yamanaka S, Woltjen K, Yamada Y.
    • Organizer
      ISSCR
    • Place of Presentation
      カナダ バンクーバー
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analyses of genomic imprinting during iPS cell derivation2014

    • Author(s)
      八木正樹、蝉克憲、Woltjen Knut、山中伸弥、山田泰広
    • Organizer
      日本発生生物学会大会
    • Place of Presentation
      愛知県
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生体内における体細胞初期化過程のDNAメチル化解析2013

    • Author(s)
      蝉克憲,大西紘太郎,田中彰人,山本拓也,山中伸弥Woltjen Knut,山田泰広
    • Organizer
      エピジェネティクス研究会
    • Place of Presentation
      奈良県
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Book] 幹細胞研究と再生医療2013

    • Author(s)
      蝉克憲、山田泰広
    • Total Pages
      238
    • Publisher
      南山堂
    • Related Report
      2013 Final Research Report
  • [Book] 遺伝子医学MOOKエピジェネティクスと病気2013

    • Author(s)
      蝉克憲、山田泰広
    • Total Pages
      288
    • Publisher
      メディカルドゥ
    • Related Report
      2013 Final Research Report
  • [Book] 幹細胞研究と再生医療2013

    • Author(s)
      蝉 克憲、山田 泰広
    • Total Pages
      238
    • Publisher
      南山堂
    • Related Report
      2013 Annual Research Report
  • [Book] 遺伝子医学MOOK エピジェネティクスと病気2013

    • Author(s)
      蝉 克憲、山田 泰広
    • Total Pages
      288
    • Publisher
      メディカル ドゥ
    • Related Report
      2013 Annual Research Report
  • [Remarks] ①生体内における体細胞の不完全な初期化はエピゲノム制御の変化による発がんを惹起する

    • URL

      http://first.lifesciencedb.jp/archives/8458

    • Related Report
      2013 Final Research Report
  • [Remarks] ②遺伝子の変異によらないがん化の仕組みを解明~iPS細胞技術の応用~

    • URL

      https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/140214-095605.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 生体内における体細胞の不完全な初期化はエピゲノム制御の変化による発がんを惹起する

    • URL

      http://first.lifesciencedb.jp/archives/8458

    • Related Report
      2013 Annual Research Report
  • [Remarks] 遺伝子の変異によらないがん化の仕組みを解明 ~iPS細胞技術の応用~

    • URL

      https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/140214-095605.html

    • Related Report
      2013 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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