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Identification of genetic factors affect the success or failure of the transformation in barley

Research Project

Project/Area Number 24880025
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionOkayama University

Principal Investigator

HISANO Hiroshi  岡山大学, その他部局等, 助教 (70415454)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物ホルモン / 再分化 / マイクロアレイ / オオムギ / 形質転換
Research Abstract

We explored the genomic regions required for genetic transformation by using a mapping populations generated from barley cv. Golden Promise, which was a reliable cultivar for transformation. As a result of this, the partial region of chromosome No. 1H, No. 2 H, No. 3H, No. 6 H and No. 7 H were estimated as the positions of candidate genes. Then we isolated 4308 genes which expression patterns were specific in Golden Promise by a comprehensive gene expression analysis using microarray. In the analysis of phytohormone, we identified that auxin showed a positive effect in regeneration of barley callus, although abscisic acid and salicylic acid showed negative.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (6 results)

All 2014 2013

All Presentation (6 results)

  • [Presentation] マイクロアレイによるオオムギカルスの網羅的な遺伝子発現解析2014

    • Author(s)
      久野裕(研究代表者)、西村秀希、佐藤和広
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      東北大学(ポスター発表)
    • Year and Date
      2014-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Microarray and hormone analysis of the calli derived from different organs and genotypes in barley2014

    • Author(s)
      Hiroshi Hisano(研究代表者), Hideki Nishimura, Takakazu Matsuura, Izumi Mori, Miki Yamane and Kazuhiro Sato
    • Organizer
      EUCARPIA Cereals Section ITMI Joint Conference
    • Place of Presentation
      ワーゲニゲローデ、ドイツ(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロアレイによるオオムギカルスの網羅的な遺伝子発現解析2014

    • Author(s)
      久野 裕、西村 秀希、佐藤 和広
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microarray and hormone analysis of the calli derived from different organs and genotypes in barley2014

    • Author(s)
      Hiroshi Hisano, Hideki Nishimura, Takakazu Matsuura, Izumi Mori, Miki Yamane and Kazuhiro Sato
    • Organizer
      EUCARPIA Cereals Section - ITMI Joint Conference
    • Place of Presentation
      Wernigerode, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオムギの異なる組織に由来するカルスのホルモン分析2013

    • Author(s)
      久野裕(研究代表者)、松浦恭和、森泉、山根美樹、佐藤和広
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学(口頭発表)
    • Year and Date
      2013-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] オオムギの異なる組織に由来するカルスのホルモン分析2013

    • Author(s)
      久野 裕、松浦 恭和、森 泉、山根 美樹、佐藤 和広
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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