Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Research Abstract |
Skunk cabbage (Symplocarpus renifolius) can keep the spadix temperature at around 20 degrees Celsius even if the air temperature falls below freezing. In the thermogenic spadix, a large amount of mitochondria was observed and the genes involved in cellular respiration or mitochondrial function were highly expressed. These results suggest that transcriptional regulation systems may exist to activate mitochondrial biogenesis during the thermogenic stage in skunk cabbage. To elucidate the transcriptional mechanisms, we examined 5' flanking regions of several genes which may play a role directly or indirectly in floral thermogenesis and was highly expressed in the thermogenic-stage spadices. As a result, we identified several consensus sequences conserved in all promoter regions in the genes examined here. These cis-regulatory elements may play a role in regulating the expression of the genes, and lead to massive floral thermogenesis through increased mitochondrial function.
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