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Regulation of bacterial metabolism by genetic engineering and electrochemical approaches

Research Project

Project/Area Number 24880030
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

KOUZUMA Atsushi  東京薬科大学, 生命科学部, 助教 (20634471)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords微生物電気化学 / 遺伝子発現 / 細胞外電子伝達 / 電気化学 / 代謝工学 / 物質生産
Research Abstract

We tried to develop a method for controlling bacterial metabolism and for producing useful chemicals by a combination of genetic engineering and electrochemical approaches. An engineered strain of Shewanella oneidensis MR-1 with the ability to utilize glucose was constructed and cultivated in electrochemical cells with different electrode potentials. The analysis of transcripts and metabolites revealed the alteration of gene expression and metabolite profiles in response to the change of electrode potentials. We also investigated the regulatory mechanisms for the genes involved in electron transfer to electrodes and identified a transcriptional activator for these genes.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (23 results)

All 2014 2013 2012

All Presentation (23 results) (of which Invited: 1 results)

  • [Presentation] 細胞外電子伝達系シトクロム遺伝子の転写活性化因子の同定2014

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 電流生成条件下におけるShewanella oneidensis変異株の糖代謝産物の同定2014

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Shewanella oneidensis MR-1変異株のグルコース代謝に伴う電流生産と代謝産物の解析2013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      第12回微生物研究会
    • Place of Presentation
      東京
    • Year and Date
      2013-10-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Shewanella oneidensisの細胞外電子伝達系遺伝子の発現に必要な転写因子の同定2013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      第12回微生物研究会
    • Place of Presentation
      東京
    • Year and Date
      2013-10-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Electrochemical selection and characterization of Shewanella oneidensis mutants with enhanced capability for electricity generation2013

    • Author(s)
      高妻篤史,大場瞳,渡邉一哉
    • Organizer
      FEMS2013
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Year and Date
      2013-07-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Electric current and metabolite production from glucose in engineered Shewanella oneidensis MR-12013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      FEMS2013
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Year and Date
      2013-07-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Differential transcriptional regulation of outer-membrane cytochromes in Shewanella oneidensis MR-12013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      FEMS2013
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Year and Date
      2013-07-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 電流生成条件下におけるShewanella oneidensis変異株の糖代謝産物の同定2013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      神奈川
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞外電子伝達系シトクロム遺伝子の転写活性化因子の同定2013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      神奈川
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞外電子伝達に関与する外膜シトクロム遺伝子の発現制御機構の解析2013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] グルコース資化能を付加したShewanella oneidensis MR-1変異株の電流生産能力の解析2013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 電気化学集積法による高電流Shewanella oneidensis MR-1変異株の単離と解析2013

    • Author(s)
      高妻篤史,大場瞳,但馬望,橋本和仁,渡邉一哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Shewanella oneidensisの細胞外電子伝達系遺伝子の発現に必要な転写因子の同定2013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      第12回微生物研究会
    • Place of Presentation
      東京電機大学東京千住キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Shewanella oneidensis MR-1変異株のグルコース代謝に伴う電流生産と代謝産物の解析2013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      第12回微生物研究会
    • Place of Presentation
      東京電機大学東京千住キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Electrochemical selection and characterization of Shewanella oneidensis mutants with enhanced capability for electricity generation2013

    • Author(s)
      高妻篤史,大場瞳,渡邉一哉
    • Organizer
      FEMS2013(第5回欧州マイクロバイオロジー会議)
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Differential transcriptional regulation of outer-membrane cytochromes in Shewanella oneidensis MR-12013

    • Author(s)
      笠井拓也,高妻篤史,渡邉一哉
    • Organizer
      FEMS2013(第5回欧州マイクロバイオロジー会議)
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Electric current and metabolite production from glucose in engineered Shewanella oneidensis MR-12013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      FEMS2013(第5回欧州マイクロバイオロジー会議)
    • Place of Presentation
      ドイツ,ライプツィヒ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Shewanellaの細胞外電子伝達機構と微生物燃料電池における電流生産2013

    • Author(s)
      高妻篤史
    • Organizer
      日本生化学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 電気化学集積法による高電流Shewanella oneidensis MR-1変異株の単離と解析2013

    • Author(s)
      高妻篤史,大場瞳,但馬望,橋本和仁,渡邉一哉
    • Organizer
      2013年度日本農芸化学会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] グルコース資化能を付加したShewanella oneidensis MR-1変異株の電流生産能力の解析2013

    • Author(s)
      中川元,高妻篤史,渡邉一哉
    • Organizer
      2013年度日本農芸化学会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞外電子伝達に関与する外膜シトクロム遺伝子の発現制御機構の解析2013

    • Author(s)
      笠井拓哉,高妻篤史,渡邉一哉
    • Organizer
      2013年度日本農芸化学会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞外電子伝達系遺伝子の発現制御機構の解析2012

    • Author(s)
      高妻篤史,笠井拓哉,渡邉一哉
    • Organizer
      第11回微生物研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞外電子伝達系遺伝子の発現制御機構の解析2012

    • Author(s)
      高妻篤史,笠井拓哉,渡邉一哉
    • Organizer
      第11回微生物研究会
    • Place of Presentation
      東京大学農学部
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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