Regulation of bacterial metabolism by genetic engineering and electrochemical approaches
| Project/Area Number |
24880030
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 微生物電気化学 / 遺伝子発現 / 細胞外電子伝達 / 電気化学 / 代謝工学 / 物質生産 |
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| Research Abstract |
We tried to develop a method for controlling bacterial metabolism and for producing useful chemicals by a combination of genetic engineering and electrochemical approaches. An engineered strain of Shewanella oneidensis MR-1 with the ability to utilize glucose was constructed and cultivated in electrochemical cells with different electrode potentials. The analysis of transcripts and metabolites revealed the alteration of gene expression and metabolite profiles in response to the change of electrode potentials. We also investigated the regulatory mechanisms for the genes involved in electron transfer to electrodes and identified a transcriptional activator for these genes.
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Report
(3 results)
Research Products
(23 results)
