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Research on the ecology of symbiotic microbes of platypodid beetles disclosed by next-generation sequencing technology

Research Project

Project/Area Number 24880034
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

ENDO Rikiya  独立行政法人理化学研究所, バイオリソースセンター, 協力研究員 (90634494)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsナラ枯れ / カシノナガキクイムシ / 養菌性キクイムシ / 次世代シーケンサー / 酵母生態 / メタゲノム / 太平洋型
Research Abstract

I disclosed the whole microbiome on the beetle galleries of a platypodid beetle ('pacific type') that vectors oak wilt disease.
Bait logs of Quercus serrata that attacked by 'pacific type' were collected and broken into small pieces including pupal chamber (n=2) or lateral gallery (n=4). Microbiome on the beetle galleries was analysed by culture dependent and/or non-dependent methods.
Population analysis by dilution plate technique (culture dependent) revealed that fungal population on the galleries of 'pacific type' was quite similar to that of Platypus quercivorus, a vector of Japanese Oak Wilt. Also, Actinomyces sp. was detected from all 6 samples tested by the microbiome analysis using a next generation sequencer (culture non-dependent), indicating that it is likely an important symbiont of 'pacific type'.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (23 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (16 results) (of which Invited: 3 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Comparative genomics of Taphrina fungi causing varying degrees of tumourous deformity in plants.2014

    • Author(s)
      Tsai IJ, Tanaka E, Masuya H, Tanaka R, Hirooka Y, Endoh R, Sahashi N, Kikuchi T.
    • Journal Title

      Genome Biology and Evolution

      Volume: (printing) Issue: 4 Pages: 861-872

    • DOI

      10.1093/gbe/evu067

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative genomics of Taphrina fungi causing varying degrees of tumorous deformity in plants2014

    • Author(s)
      Tsai IJ, Tanaka E, Masuya H, Tanaka R, Hirooka Y, Endoh R, Sahashi N, Kikuchi T
    • Journal Title

      Genome Biology and Evolution

      Volume: 6(4) Issue: 1 Pages: 861-872

    • DOI

      10.1038/srep04776

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production2014

    • Author(s)
      Tanimura A, Takashima M, Sugita T, Endoh R, Kikukawa M, Yamaguchi S, Sakuradani E, Ogawa J, Shima J
    • Journal Title

      Bioresource Technology

      Volume: 153 Pages: 230-235

    • DOI

      10.1016/j.biortech.2013.11.086

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomic richness of yeasts in Japan within subtropical and cool temperature areas2012

    • Author(s)
      Takashima M, Sugita T, Van BH, Nakamura M, Endoh R, Ohkuma M
    • Journal Title

      PLos One

      Volume: 11 Issue: 11 Pages: e50784-e50784

    • DOI

      10.1371/journal.pone.0050784

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Yeasts Associated with Coleopteran Beetles in Forest2012

    • Author(s)
      遠藤力也
    • Journal Title

      Journal of the Japanese Forest Society

      Volume: 94 Issue: 6 Pages: 326-334

    • DOI

      10.4005/jjfs.94.326

    • NAID

      130003369352

    • ISSN
      1349-8509, 1882-398X
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 和歌山県産ナガキクイムシPlatypus sp.の坑道の菌類群集2014

    • Author(s)
      遠藤力也、升屋勇人、大熊盛也
    • Organizer
      日本森林学会第125回大会
    • Place of Presentation
      大宮ソニックシティ(大宮)
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] ナガキクイムシ共生酵母の生理生化学的特徴と分子系統2014

    • Author(s)
      遠藤 力也、大熊 盛也
    • Organizer
      日本菌学会第58回大会
    • Place of Presentation
      サイエンスヒルズこまつ(石川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 森に酵母資源の宝庫を見つける2013

    • Author(s)
      遠藤力也、升屋勇人、大熊盛也
    • Organizer
      日本微生物資源学会第20回大会
    • Place of Presentation
      つくば国際会議場(つくば)
    • Year and Date
      2013-06-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 和歌山県産ナガキクイムシPlatypus sp.の坑道の菌類群集2013

    • Author(s)
      遠藤力也、升屋勇人、大熊盛也
    • Organizer
      日本菌学会第57回大会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2013-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 森の虫に有用酵母の故郷を見つける2013

    • Author(s)
      遠藤力也
    • Organizer
      第5回新産業酵母研究会講演会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2013-05-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Yeasts associated with Coleopteran beetles in Japan2013

    • Author(s)
      Endoh R, Ohkuma M
    • Organizer
      The 13th International Conference of Culture Collections (ICCC 13)
    • Place of Presentation
      北京(中華人民共和国)
    • Related Report
      2013 Final Research Report
  • [Presentation] Yeasts associated with Coleopteran beetles in Japan2013

    • Author(s)
      Rikiya Endoh, Moriya Ohkuma
    • Organizer
      The 13th International Conference of Culture Collections
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Annual Research Report
  • [Presentation] 森の虫に有用酵母の故郷を見つける2013

    • Author(s)
      遠藤力也
    • Organizer
      第5回 新産業酵母研究会 講演会
    • Place of Presentation
      東京大学農学部 生命科学研究科キャンパス
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 和歌山県産ナガキクイムシ Platypus sp. の坑道の菌類群集構造2013

    • Author(s)
      遠藤力也
    • Organizer
      第57回 日本菌学会大会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 森に酵母資源の宝庫を見つける2013

    • Author(s)
      遠藤力也
    • Organizer
      日本微生物資源学会 第20回大会
    • Place of Presentation
      茨城県つくば市 つくば国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酵母生態学に挑む、筑波大学プレ戦略イニシアティブ「生物機能の高度利用を目指した応用微生物学研究拠点」シンポジウムつくばの応用微生物研究~理研BRC-JCM2012

    • Author(s)
      遠藤力也
    • Organizer
      産総研
    • Place of Presentation
      筑波大学(つくば)
    • Year and Date
      2012-12-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 和歌山県産ナガキクイムシPlatypus sp.の坑道の菌類群集

    • Author(s)
      遠藤 力也、升屋 勇人、大熊 盛也
    • Organizer
      日本森林学会第125回大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 森に酵母資源の宝庫を見つける

    • Author(s)
      遠藤 力也、升屋 勇人、大熊 盛也
    • Organizer
      日本微生物資源学会第20回大会
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 和歌山県産ナガキクイムシPlatypus sp.の坑道の菌類群集

    • Author(s)
      遠藤 力也、升屋 勇人、大熊 盛也
    • Organizer
      日本菌学会第57回大会
    • Place of Presentation
      東京農業大学(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 森の虫に有用酵母の故郷を見つける

    • Author(s)
      遠藤 力也
    • Organizer
      第5回新産業酵母研究会講演会
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 森に微生物資源の宝庫を見つける~小さな住人たちの大きなゆりかご~

    • Author(s)
      遠藤 力也
    • Organizer
      東京大学森林科学専攻 森林科学セミナー
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 実践有用微生物培養のイロハ(片倉啓雄編)第5章継代と保存2014

    • Author(s)
      遠藤力也、髙島昌子
    • Publisher
      (株)エヌ・ティー・エス(印刷中)
    • Related Report
      2013 Final Research Report
  • [Remarks] ①日本微生物資源学会第20回大会(〔学会発表〕③)において優秀発表賞受賞

    • Related Report
      2013 Final Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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