| Project/Area Number |
24880035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KATO Souichiro 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (30597787)
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|---|
| Project Period (FY) |
2012-08-31 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 微生物 / 酢酸分解 / メタン生成 / モデル共生系 / アンモニア阻害 / 高圧 / 酢酸 / 高圧力 / 共培養 |
|---|
| Research Abstract |
Methanogenic acetate degradation proceeds via two different pathways, aceticlastic and syntrophic pathways. Here we studied the effects of ammonia and high-pressure stresses on the two methanogenic pathways using model microbial consortia. Ammonia stress induced direct inhibition of methanogenic enzymes, disruption of intrasellular pH homeostasis, and oxidative stresses to the microbes tested, and inhibited aveticlastic methanogenesis more strongly. On the other hand, high pressure stress suppressed syntrophic pathway more strongly. We found reconstruction of denatured proteins and reconstruction of cell membrane/wall are required to generate methane under high-pressure conditions.
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