Research Project
Grant-in-Aid for Research Activity Start-up
The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.
All 2014 2013 2012 Other
All Journal Article (9 results) (of which Peer Reviewed: 9 results, Open Access: 1 results) Presentation (20 results) Remarks (2 results)
Mol. Cell
Volume: 53(3) Pages: 393-406
Biochem Biophys. Res. Commun
Volume: 443(3) Pages: 975-979
120007130532
Molecular cell
Volume: 53 Issue: 3 Pages: 393-406
10.1016/j.molcel.2014.01.009
Biochemical and Biophysical Research Communications
Volume: 443 Issue: 3 Pages: 975-979
10.1016/j.bbrc.2013.12.071
Front. Microbiol
Volume: 4 Pages: 398-398
120007130538
Frontiers in Microbiology
PLoS One
Volume: 7 Issue: 8 Pages: 1-15
10.1371/journal.pone.0043031
J. Virol
Volume: 86(20) Pages: 11086-11095
120007130530
Journal of Virology
Volume: 86 Issue: 20 Pages: 11086-11095
10.1128/jvi.00453-12
http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/
http://www.md.tsukuba.ac.jp/basic-med/kawaguchilab/