| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
|
|---|
| Research Abstract |
In transplants of tissue engineering, the surrounding fibrous tissues form which are speculated to support the tissue regeneration. Adhesion molecule periostin (PN) is abundantly localized in surrounding fibrous tissues, it may be essential for tissue regeneration. To verify this, we investigated the phenotype of cartilage regeneration, using wild-type (Pn+/+) or PN knockout (Pn-/-) mice. When cubic sponge containing Pn+/+ chondrocytes were syngenically transplanted in Pn+/+ mice, the cubic cartilage was regenerated with abundant cartilage matrix. While in Pn-/- mice, the surrounding tissues were devoid of PN, exhibiting irregular shapes of transplants. In vitro assay, PN contained in the culture medium failed to show any effects, although the 3D culture in collagen gel premixed with PN enhanced chondroycte differentiation, suggesting that biological action of PN could be collagen-mediated.
|
