Role of AID as a demethylating enzymes in reactivation of chronic viral hepatits

• Project/Area Number: 24890075
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kanazawa University
• Principal Investigator: WAKAE kousho 金沢大学, 医学系, 助教 (70638303)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: AID/APOBEC / HBV / 脱メチル化 / 慢性感染 / B型肝炎再活性化 / 肝炎再活性化 / cccDNA

Research Abstract

We aimed to verify whether AID/APOBEC family could demethylate covalently-closed-circular DNA (cccDNA) of human Hepatitis B Virus (HBV). As an alternative experimental system, we utilized chicken hepatocyte cell line that stably express duck Hepatitis B Virus (DHBV) gene. The result suggested that APOBEC3G demethylate pre-core region of DHBV cccDNA.
We applied the hypothesis to Human Papilloma Viruses (HPVs), that possess circular double-strand DNA. This was supported by the experiment using a HPV16-positive cervical cell line.

Research Products

• [Journal Article] APOBEC3 deaminases induce hypermutation in human papillomavirus 16 DNA upon beta interferon stimulation (2014)
  • Author(s): Wang Z, Wakae K, Kitamura K, Aoyama S, Liu G, Koura M, Monjurul AM, Kukimoto I, Muramatsu M
  • Journal Title: J Virol. Volume: 88(2) Pages: 1308-17
  • NAID: 120005368221
  • Peer Reviewed
• [Journal Article] APOBEC3 deaminases induce hypermutation in human papillomavirus 16 DNA upon beta interferon stimulation. (2014)
  • Author(s): Wang Z, Wakae K, Kitamura K, Aoyama S, Liu G, Koura M, Monjurul AM, Kukimoto I, Muramatsu M.
  • Journal Title: Journal of Virology Volume: 88 Issue: 2 Pages: 1308-1317
  • DOI: 10.1128/jvi.03091-13
  • NAID: 120005368221
  • URL: http://dspace.lib.kanazawa-u.ac.jp/dspace/handle/2297/36486
  • Peer Reviewed
• [Journal Article] RNA editing of hepatitis B virus transcripts by activation-induced cytidine deaminase (2013)
  • Author(s): Liang G, Kitamura K, Wang Z, Liu G, Chowdhury S, Fu W, Koura M, Wakae K, Honjo T, Muramatsu M
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110(6) Pages: 2246-51
  • NAID: 120005253841
  • Peer Reviewed
• [Journal Article] RNA editing of hepatitis B virus transcripts by activation-induced cytidine deaminase. (2013)
  • Author(s): Liang, G., K. Kitamura, Z. Wang, G. Liu, S. Chowdhury, W. Fu, M. Koura, K. Wakae, T. Honjo, and M. Muramatsu
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Issue: 6 Pages: 2246-2251
  • DOI: 10.1073/pnas.1221921110
  • NAID: 120005253841
  • URL: http://dspace.lib.kanazawa-u.ac.jp/dspace/handle/2297/34139
  • Peer Reviewed
• [Presentation] APOBEC3はHPVゲノムにhypermutationを導入する (2013)
  • Author(s): 若江亨祥
  • Organizer: 第31回生化学会北陸支部大会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2013-05-25
• [Presentation] POBEC3 hypermutates human papillomavirus (HPV) 16 genome (2013)
  • Author(s): Kousho Wakae, Satoru Aoyama, Wang Zhe, Liu Guangyan, Ahsan Monjurul, Mieko Imayasu, Miki Koura, Kouichi Kitamura, Mitsuhiro Nakamura, Satoru Kyo, Satoru Kondo, Tomokazu Yoshizaki, Iwao Kukinmoto, Tomoaki Nishiyama, Masamichi Muramatsu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場・神戸ポートピアホテル
• [Presentation] APOBEC3はHPVゲノムにhypermutationを導入する (2013)
  • Author(s): 若江亨祥,青山慧,王哲,喜多村晃一,劉光炎,アハサン=モハメド=モンジュルル,中村充宏,京哲,柊元巌,西山智明,村松正道
  • Organizer: NGS現場の会・第三回研究会
  • Place of Presentation: 神戸国際会議場
• [Presentation] APOBEC3はHPVゲノムに hypermutationを導入する (2013)
  • Author(s): 若江 亨祥
  • Organizer: 第31回生化学会 北陸支部大会
  • Place of Presentation: 金沢大学角間キャンパス
• [Presentation] APOBEC3はHPVゲノムに hypermutationを導入する (2013)
  • Author(s): 若江 亨祥, 青山 慧, 王 哲, 喜多村 晃一, 劉 光炎, アハサン=モハメド=モンジュルル, 中村 充宏, 京 哲, 柊元 巌, 西山 智明, 村松 正道
  • Organizer: NGS現場の会・第三回研究会
  • Place of Presentation: 神戸国際会議場
• [Presentation] APOBEC3 hypermutates human papillomavirus (HPV) 16 genome (2013)
  • Author(s): Kousho Wakae, Satoru Aoyama, Wang Zhe, Liu Guangyan, Ahsan Monjurul, Mieko Imayasu, Miki Koura, Kouichi Kitamura, Mitsuhiro Nakamura, Satoru Kyo, Satoru Kondo, Tomokazu Yoshizaki, Iwao Kukinmoto, Tomoaki Nishiyama, Masamichi Muramatsu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場・神戸ポートピアホテル
• [Presentation] APOBEC proteins introduce hypermutation in Human papillomavirus-16(HPV-16) viral DNA triggered by IFN stimulation (2012)
  • Author(s): 若江亨祥
  • Organizer: The 4^<th> Japan-Korea Joint Symposium on Recent Advance in Medical Science
  • Place of Presentation: 金沢大学医学部記念館
  • Year and Date: 2012-11-07
• [Presentation] APOBEC proteins introduce hypermutation in Human papillomavirus 16(HPV-16) viral DNA triggered by IFN stimulation
  • Author(s): 若江 亨祥
  • Organizer: The 4th Japan-Korea Joint Symposium on Recent Advance in Medical Science
  • Place of Presentation: 金沢大学医学部記念館