Functional analysis of human profilaggrin C-terminus region: Identification of molecular mechanism in profilaggrin processing
| Project/Area Number |
24890082
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | C末端領域の機能 / C末端領域結合タンパク / 蛋白のプロセシング / 表皮バリア機能 / フィラグリン / タンパク精製 |
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| Research Abstract |
Filaggrin monomers are produced through profilaggrin (FLG) processing, and they are considered to be crucial in epidermal barrier function. Recently, lack of FLG C-terminus region in filaggrin gene mutation carriers was shown to be associated with significant reduction in filaggrin monomer production. To this end, we sought to evaluate the function of FLG C-terminus region in FLG processing. 1) Three types of C-terminus expression vectors were constructed, and these constructs were transfected to COS-7 cells followed by RT-PCR and Western blot analysis. 2) By pull-down assay using FLG C-terminus protein, molecules with interacting potential were searched. As result, 1) deletion of FLG C-terminus region did not alter the FLG mRNA/protein expression, suggesting that FLG C-terminus region is possibly involved in the processing of FLG, and not with the production of FLG itself. 2) Candidate proteins that interact with FLG C-terminus region were identified, such as galectin-7, etc.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] A group of atopic dermatitis without IgE elevation or barrier impairment shows a high Th1 frequency: Possible immunological state of the intrinsic type2012
Author(s)
Kabashima-Kubo R, Nakamura M, Sakabe, J, Sigita K, Hino R, Mori T, Kobayashi M, Bito T, Kabashima K, Ogasawara K, Nomura Y, Nomura T, Akiyama M, Shimizu H, Tokura Y.
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Journal Title
J Dermatol Sci
Volume: 67
Issue: 1
Pages: 37-43
DOI
Related Report
Peer Reviewed
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