Identification of receptors for phosphacan which inhibit axonal regeneration
| Project/Area Number |
24890084
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
SAKAMOTO Kazuma 名古屋大学, 高等研究院(医), 特任助教 (60612801)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | プロテオグリカン / コンドロイチン硫酸 / ケラタン硫酸 / 神経軸索 / 再生 / 軸索再生 |
|---|
| Research Abstract |
Highly purified recombinant phosphacan was purepared using anion exchange and gel filtration chromatography. Increasing gradient of recombinant phosphacan induced dystrophic endball, a morphological marker for regeneration stopping axons, to cultured dorsal root ganglion neurons. Using electron microscope and immunocytochemistry, autophagosomes were found in dystrophic endball, suggesting that autophagy is an essential cellular event for formation of dystrophic endball.
Surface plasmon resonance assay revealed that phosphacan strongly bound to PTPsigma and LAR which are known as receptors for chondroitin sulfate. Surprisingly, interaction between phosphacan and PTPsigma or LAR was two-phase manner, and not only chondroitin sulfate but also keratan sulfate were involved in interaction. Free keratan sulfate sugar also bound to PTPsigma and LAR.
PTPzeta, another member of receptors tyrosine phosphatase, was also identified to be involved in inhibition of axonal regeneration.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS.2013
Author(s)
Kenichi Hirano, Tomohiro Ohgomori, Kazuyoshi Kobayashi, Fumiaki Tanaka, Tomohiro Matsumoto, Takamitsu Natori, Yukihiro Matsuyama, Kenji Uchimura, Kazuma Sakamoto, Hideyuki Takeuchi, Akihiro Hirakawa, Akio Suzumura, Gen Sobue, Naoki Ishiguro, Shiro Imagama, Kenji Kadomatsu
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Journal Title
PLoS ONE
Volume: 8(6)
Issue: 6
Pages: e66969-e66969
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Minocycline selectively inhibits M1 polarization of microglia2013
Author(s)
Kobayashi K, Imagama S, Ohgomori T, Hirano K, Uchimura K, Sakamoto K, Hirakawa A, Takeuchi H, Suzumura A, Ishiguro N, Kadomatsu K
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Journal Title
Cell Death Dis.
Volume: 4
Related Report
Peer Reviewed
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[Presentation] 未定2014
Author(s)
坂元一真 門松健治
Organizer
第87回日本生化学会大会
Place of Presentation
国立京都国際会館・京都市
Related Report
Invited
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