| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
In the present study, we focused on the lincRNAs, a non-coding RNA, which may be involved in cardiac hypertrophy. We screened lincRNAs which were upregulated in mouse heart subjected to transverse aortic constriction, and identified three lincRNAs. In the present study, we tried to clarify the functions of the lincRNAs. Firstly, we overexpressed the lincRNAs in neonatal mouse cardiomyocytes, and found that cardiomyocyte hypertrophy was induced. Also, we demonstrated that ANP and BNP, markers of cardiac hypertrophy, were significantly increased when the two lincRNAs were overexpressed. Furthermore, we sought to evaluate whether a lincRNA bound to microRNAs. We found that lincRNA could bind to some microRNAs that play inhibitory role to hypertrophy. Finally, we revealed that protein level of cyclin was significantly increased in neonatal cardiomyocytes overexpressed the lincRNA. These results indicate that lincRNAs may be involved in cardiac hypertrophy.
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