Exploration of a new biomarker for predicting prostate cancer prognosis using xenograft model

• Project/Area Number: 24890098
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: TERADA Naoki 京都大学, 医学(系)研究科(研究院), 助教 (60636637)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 前立腺癌 / バイオマーカー / xenograft

Research Abstract

We have established four kinds of patient derived tumor tissue (PDTT) xenograft model of prostate cancer (KUCaP-1,-2,-3,-4). Our aim was to identify new biomarker for prostate cancer using PDTT xenograft model. We especially focused on KUCaP-3, which forms cyst containing high density tumor origin proteins. High sensitivity mass spectrometry analysis identified some kinds of biomarker candidate proteins. We are planning to investigate their function.

Research Products

• [Journal Article] An EP4 Antagonist ONO-AE3-208 Suppresses Cell Invasion, Migration, and Metastasis of Prostate Cancer (2014)
  • Author(s): Xu S, Zhang Z, Ogawa O, Yoshikawa T, Sakamoto H, Shibasaki N, Goto T, Wang L, Terada N
  • Journal Title: Cell Biochem Biophys. Volume: (Epub ahead of print)
• [Journal Article] Correlation of Sprouty1 and Jagged1 with Aggressive Prostate Cancer Cells with Different Sensitivities to Androgen Deprivation (2014)
  • Author(s): Terada N, Shiraishi T, Zeng Y, Aw-Yong KM, Liu Z, Takahashi S, Luo J, LupoldSE, Kulkarni P, Getzenberg RH
  • Journal Title: J Cell Biochem. Volume: (Epub ahead of print) Issue: 9 Pages: 1505-1515
  • DOI: 10.1002/jcb.24805
• [Journal Article] The expression profile of phosphatidylinositol in high spatial resolution imaging mass spectrometry as a potential biomarker for prostate cancer (2014)
  • Author(s): Goto T, Terada N, Inoue T, Nakayama K, Okada Y, Yoshikawa T, Miyazaki Y, Uegaki M, Sumiyoshi S, Kobayashi T, Kamba T, Yoshimura K, Ogawa O
  • Journal Title: PLoS One Volume: 9(2) Issue: 2 Pages: e90242-e90242
  • DOI: 10.1371/journal.pone.0090242
  • Peer Reviewed
• [Journal Article] Novel constant-pressure irrigation technique for the treatment of renal pelvic tumors after ipsilateral ureterectomy (2014)
  • Author(s): Nakamura K, Terada N, Sugino Y, Yamasaki T, Matsui Y, Imamura M, Okubo K, Kamba T, Yoshimura K, Ogawa O
  • Journal Title: Int J Urol. Volume: (Epub ahead of print) Issue: 6 Pages: 617-618
  • DOI: 10.1111/iju.12386
  • NAID: 120005647475
• [Journal Article] A case of prostate carcinosarcoma successfully treated with combined modality therapy (2013)
  • Author(s): Matsuoka T, Sugino Y, Kobayashi T, Terada N, Yamasaki T, Matsui Y, Imamura M, Okubo K, Kamba T, Yoshimura K, Ogawa O
  • Journal Title: Hinyokika Kiyo Volume: 59(11) Pages: 749-52
  • NAID: 120005350248
• [Journal Article] Stress-response protein RBM3 attenuates the stem-like properties of prostate cancer cells by interfering with CD44 variant splicing (2013)
  • Author(s): Zeng Y, Wodzenski D, Gao D, Shiraishi T, Terada N, Li Y, Vander Griend DJ, Luo J, Kong C, Getzenberg RH, Kulkarni P
  • Journal Title: Cancer Res. Volume: 73(13) Issue: 13 Pages: 4123-33
  • DOI: 10.1158/0008-5472.can-12-1343
• [Journal Article] Acquisition of paclitaxel resistance is associated with a more aggressive and invasive phenotype in prostate cancer (2013)
  • Author(s): Kim JJ, Yin B, Christudass CS, Terada N, Rajagopalan K, Fabry B, Lee DY, Shiraishi T, Getzenberg RH, Veltri RW, An SS, Mooney SM
  • Journal Title: J Cell Biochem. Volume: 114(6) Issue: 6 Pages: 1286-93
  • DOI: 10.1002/jcb.24464
• [Journal Article] Overexpression of ETS-1 is associated with malignant biological features of prostate cancer (2012)
  • Author(s): Li B, Shimizu Y, Kobayashi T, Terada N, Yoshimura K, Kamba T, Mikami Y, Inoue T, Nishiyama H, Ogawa O
  • Journal Title: Asian J Androl. Volume: 14(6) Issue: 6 Pages: 860-3
  • DOI: 10.1038/aja.2012.107
  • NAID: 120005289841
• [Journal Article] Cyr61 is a potential prognostic marker for prostate cancer (2012)
  • Author(s): Terada N, Kulkarni P, Getzenberg RH
  • Journal Title: Asian J Androl. Volume: 14(3) Issue: 3 Pages: 405-8
  • DOI: 10.1038/aja.2011.149
• [Journal Article] Cyr61 is regulated by cAMP-dependent protein kinase with serum levels correlating with prostate cancer aggressiveness (2012)
  • Author(s): Terada N, Shiraishi T, Zeng Y, Mooney SM, Yeater DB, Mangold LA, Partin AW, Kulkarni P, Getzenberg RH
  • Journal Title: Prostate Volume: 72(9) Issue: 9 Pages: 966-76
  • DOI: 10.1002/pros.21501
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織のin situ脂質プロファイリング (2014)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎有、植垣正幸、吉川武志、小林恭、神波大己、吉村耕治、小川修
  • Organizer: 第23回泌尿器科分子細胞研究会
  • Place of Presentation: 山形(P-05)
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2014)
  • Author(s): Takayuki Goto, Naoki Terada, Takahiro Inoue, Kenji Nakayama, Yu Miyazaki, Masayuki Uegaki, Takeshi Yoshikawa, Takashi Kobayashi, Tomomi Kamba, Koji Yoshimura, Osamu Ogawa
  • Organizer: The 4th Congress of Asian Pacific Prostate Society
  • Place of Presentation: 沖縄(P-105)
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2014)
  • Author(s): Takayuki Goto, Naoki Terada, Takahiro Inoue, Kenji Nakayama, Yu Miyazaki, Masayuki Uegaki, Takeshi Yoshikawa, Takashi Kobayashi, Tomomi Kamba, Koji Yoshimura, Osamu Ogawa
  • Organizer: The 4th Congress of Asian Pacific Prostate Society
  • Place of Presentation: 沖縄
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織のin situ 脂質プロファイリング (2014)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎有、植垣正幸、吉川武志、小林恭、神波大己、吉村耕治、小川修
  • Organizer: 第23回泌尿器科分子細胞研究会
  • Place of Presentation: 山形
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2013)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎有、植垣正幸、吉川武志、小林恭、清水洋祐、神波大己、吉村耕治、小川修
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜(P-3297)
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2013)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎 有、植垣正幸、吉川武志、小林恭、清水洋祐、神波大己、吉村耕治、小川修
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 臨床検体由来のマウス異種移植片を用いた、蛋白質量分析による前立腺癌新規バイオマーカーの同定 (2013)
  • Author(s): 吉川武志、赤松秀輔、小堀豪、後藤崇之、岡田能幸、清水洋祐、松井喜之、神波大己、田中祥徳、井上貴博、小川修
  • Organizer: 第101回日本泌尿器科学会総会
  • Place of Presentation: 札幌
• [Presentation] 臨床検体由来のマウス異種移植片を用いた、蛋白質量分析による前立腺癌新規バイオマーカーの同定 (2013)
  • Author(s): 吉川武志
  • Organizer: 第22回泌尿器科分子細胞研究会
  • Place of Presentation: ホテル日航高知旭ロイヤル
• [Presentation] A Proteomic Approach Using a Patient Derived Xenograft Model for Identification of a Novel Biomarker in Prostate Cancer (2012)
  • Author(s): 吉川武志
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/