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Mouse experimental myopia model and cell-type specific NGS profiling

Research Project

Project/Area Number 24890100
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKyoto University

Principal Investigator

GOTOH NORIMOTO  京都大学, 医学(系)研究科(研究院), 准教授 (20632095)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords眼細胞生物学 / 眼生化学・分子生物学 / 眼遺伝学 / 眼科 / 神経科学 / ゲノム医学 / 国際情報交換(アメリカ) / 国際研究交流(アメリカ)
Research Abstract

Myopia is a common ocular status in Japanese population, whose prevalence seems to be more than 40%. High myopia, also known as pathologic myopia, is one of the top vision threatening diseases in Northeastern Asian countries. It was reported that the neural signal enhancing myopia is originated at the inner layer of retina, but it is not clear which cell type has a largest contribution for the myopic signal. In this study, mouse ganglion cell was dissociated and enriched with flow-cytometry (FACS), and the extracted RNA was optimized for RNA Seq, performed with next generation sequencer (NGS). This approach can be applied for experimental myopic mice for revealing the pathomechanism of myopia.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (3 results)

All 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] Association between ZIC2, RASGRF1, and SHISA6 genes and high myopia in Japanese subjects2013

    • Author(s)
      Oishi M, Yamashiro K, Miyake M, Akagi-Kurashige Y, Kumagai K, Nakata I, Nakanishi H, Yoshikawa M, Oishi A, Gotoh N, Tsujikawa A; Nagahama Study Group, Yamada R, Matsuda F, Yoshimura N
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 54 Pages: 7492-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Association between ZIC2, RASGRF1, and SHISA6 genes and high myopia in Japanese subjects.2013

    • Author(s)
      Oishi M, Yamashiro K, Miyake M, Akagi-Kurashige Y, Kumagai K, Nakata I, Nakanishi H, Yoshikawa M, Oishi A, Gotoh N, Tsujikawa A; Nagahama Study Group, Yamada R, Matsuda F, Yoshimura N.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 54 Issue: 12 Pages: 7492-7497

    • DOI

      10.1167/iovs.13-12825

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the WNT7B gene provides the mechanism underlying myopia development

    • Author(s)
      Miyake M, Yamashiro K, Tabara Y, Suda K, Morooka S, Nakanishi H, Khor CC, Chen P, Nakata I, Akagi-Kurashige Y, Gotoh N, Tsujikawa A, the Nagahama Study Group, Meguro A, Kusuhara S, Polasek O, Hayward C, Wright AF, Campbell H, Richardson AJ, Schache M, Takeuchi M, Mackey DA, Hewitt AW, Cuellar G, Shi YI, Huang L, Yang Z, Leung KH, KaoPY, Yap MK, Yip SP, Moriyama M, Ohno-Matsui K, Mizuki N, MacGregor S, Vitart V, Saw M, Tin A, Tai ES, Wong TY, Cheng CY, Baird PN, Yamada R, Matsuda F, and Yoshimura N
    • Related Report
      2013 Final Research Report
    • Peer Reviewed

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Published: 2012-11-27   Modified: 2019-07-29  

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