| Project/Area Number |
24890103
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MOTOOKA Daisuke 大阪大学, 微生物病研究所, 特任研究員(常勤) (10636830)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | メタゲノム / 次世代シークエンサー / 感染症 / メタゲノム解析 / 感染症診断 / バイオインフォマティクス |
|---|
| Research Abstract |
Metagenomic analysis using 2nd generation sequencers is now widely used for comprehensive detection of pathogens in infectious diseases. This method with a single common protocol in clinical specimens doesn't depend on the type of microorganisms, overcoming the limitations of traditional methods. However, because this method detects only a part of genome information of pathogen, we can't get enough information to prevent infection disease. Here, I developed the method of infection diagnosis using 3rd generation sequencing. Third generation sequencing easily achieves higher degree of accuracy, less biased depth of coverage and a smaller number of contigs than other sequencing. The highly accurate complete genome analysis would develop the future genome epidemiology of pathogens.
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