Development of infection diagnosis using 3rd generation sequencing
| Project/Area Number |
24890103
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| Research Category |
Grant-in-Aid for Research Activity start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MOTOOKA Daisuke 大阪大学, 微生物病研究所, 特任研究員(常勤) (10636830)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | メタゲノム / 次世代シークエンサー / 感染症 / メタゲノム解析 / 感染症診断 / バイオインフォマティクス |
|---|
| Research Abstract |
Metagenomic analysis using 2nd generation sequencers is now widely used for comprehensive detection of pathogens in infectious diseases. This method with a single common protocol in clinical specimens doesn't depend on the type of microorganisms, overcoming the limitations of traditional methods. However, because this method detects only a part of genome information of pathogen, we can't get enough information to prevent infection disease. Here, I developed the method of infection diagnosis using 3rd generation sequencing. Third generation sequencing easily achieves higher degree of accuracy, less biased depth of coverage and a smaller number of contigs than other sequencing. The highly accurate complete genome analysis would develop the future genome epidemiology of pathogens.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Complete Genome Sequence of Ureaplasma parvum Serovar 3 Strain SV3F4, Isolated in Japan.2014
Author(s)
Wu HN, Nakura Y, Motooka D, Nakamura S, Nishiumi F, Ishino S, Kawai Y, Tanaka T, Takeuchi M, Nakayama M, Fujita T, Yanagihara I
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Journal Title
Genome Announc.
Volume: 2(3)
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Comprehensive metagenomic approach for detecting causative microorganisms in culture-negative infective endocarditis2014
Author(s)
Imai A, Gotoh K, Asano Y* (corresponding author), Yamada N, Motooka D, Fukushima M, Kanzaki M, Ohtani T, Sakata Y, Nishi H, Toda K, Sawa Y, Komuro I, Horii T, Iida T, Nakamura S, Takashima S.
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Journal Title
Int J Cardiol
Volume: 172
Pages: 288-289
DOI
Related Report
Peer Reviewed
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[Journal Article] Severe Bronchiolitis by human metapneumovirus coinfected with S pneumoniae.2014
Author(s)
Seki M, Yoshida H, Gotoh K, Hamada N,Motooka D, Nakamura S, Yamamoto N, Hamaguchi S, Akeda Y, Watanabe H, Iida T, and Tomono K.
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Journal Title
Resp Med Case Rep
Volume: 12
Pages: 13-15
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Severe respiratory failure due to co-infection with human metapneumovirus and Streptococcus pneumoniae2014
Author(s)
Seki M, Yoshida H, Gotoh K, Hamada N, Motooka D, Nakamura S, Yamamoto N, Hamaguchi S, Akeda Y, Watanabe H, Iida T, Tomono K
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Journal Title
Respiratory Medicine Case Reports
Volume: 12
Pages: 13-15
Related Report
Peer Reviewed
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