Regulation of Bcl-2 protein family prtoteins by virus infection

• Project/Area Number: 24890105
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: OKAMOTO Toru 大阪大学, 微生物病研究所, 助教 (80628595)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ウイルス / 細胞死 / アポトーシス / Bcl-2

Research Abstract

Japanese Encephalitis virus (JEV) infection was found to degrade Mcl1, which is a prosurvival Bcl-2 protein family, through a proteasome dependent mechanism. Bcl-x deficient human cancer cell lines were induced cell death by JEV infection. On the other hands, Mcl1 deficiency was not induced cell death. These data suggested that JEV-infected cells were dependent on Bcl-x for their survive.

Research Products

• [Journal Article] Enhanced Mcl1 stability can blunt stress-induced apoptosis, cause male sterility and promote tumorigenesis (2014)
  • Author(s): Okamoto T, Coultas L, Metcalf D, van Delft M, Glasser SP, Takiguchi M, Strasser A, Bouillet P, Adams JM, Hunag DCS
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Pages: 261-266
  • Peer Reviewed
• [Journal Article] Stabilizing the Pro-Apoptotic BimBH3 Helix (BimSAHB) Does Not Necessarily Enhance Affinity or Biological Activity (2013)
  • Author(s): Okamoto T, Zobel K, Fedorova A, Quan C, Yang H, Fairbrother WJ, Huang DC, Smith BJ, Deshayes K, Czabotar PE
  • Journal Title: ACS Chemical Biology Volume: 8 Pages: 297-302
  • Peer Reviewed
• [Journal Article] Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. (2013)
  • Author(s): Hiroshi Katoh
  • Journal Title: Journal of Virology Volume: 87(1) Issue: 1 Pages: 489-502
  • DOI: 10.1128/jvi.02186-12
  • Peer Reviewed
• [Journal Article] Japanese Encephalitis Virus Core Protein Inhibits Stress Granule Formation through an Interaction with Caprin-1 and Facilitates Viral Propagation (2012)
  • Author(s): Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, Kamitani W, Matsuura Y
  • Journal Title: Jounal of Virology Volume: 87 Pages: 489-502
  • Peer Reviewed
• [Journal Article] Sheeppox Virus SPPV14 Encodes a Bcl-2-Like Cell Death Inhibitor That Counters a Distinct Set of Mammalian Proapoptotic Proteins (2012)
  • Author(s): Okamoto T, Campbell S, Mehta N, Thibault J, Colman PM, Barry M, Huang DC, Kvansakul M
  • Journal Title: Journal of Virology Volume: 86 Pages: 11501-11511
  • Peer Reviewed