Mechanism of antiviral responses by type-II Interferon

• Project/Area Number: 24890106
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: SASAI Miwa 大阪大学, 微生物病研究所, 助教 (30631551)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 感染防御 / ウイルス / ウイルス感染 / 生体防御機構

Research Abstract

Viral infections occur at mucosal surfaces. It is important to prevent viral infection at mucosal surfaces because it is initial place. Herpes simplex virus (HSV) infect at mucosal surfaces of oral, ocular and genital. It has been reported that the protection against HSV infection are type-II interferon (IFN-gamma) dependently, especially secondary infection. However, the molecular mechanism is completely unclear. In this work, we examine the molecular mechanism of IFN-gamma mediated anti-viral immune responses, especially HSV infection. We did microarray analysis to identify gene that is involved in antiviral immune responses against HSV infection using mouse primary keratinocytes and we successfully identified the candidate molecules those are involved in suppress viral replication IFN-gamma dependently. Theses data highly assumed that this is a new mechanism of antiviral immune responses that is regulated by IFN-gamma.

Research Products

• [Journal Article] Role of Mouse and Human Autophagy Proteins in IFN-γ-Induced Cell-Autonomous Responses against Thxoplasma gondii (2014)
  • Author(s): Jun Ohshima, Youngae Lee, Miwa Sasai, Tatsuya Saitoh, Ji Su Ma, Naganori Kamiyama, Yoshiharu Matsuura, Suh Pann-Ghill, Mikako Hayashi, Shigeyuki Ebisu, Kiyoshi Takeda, Shizuo Akira, and Masahiro Yamamoto.
  • Journal Title: The Journal of Immunology Volume: 192(7) Issue: 7 Pages: 3328-35
  • DOI: 10.4049/jimmunol.1302822
  • Peer Reviewed / Open Access
• [Journal Article] Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells. (2014)
  • Author(s): Ono C., Ninomiya A., Yamamoto S., Abe T., Wen X., Fukuhara T., Sasai M., Yamamoto M., Saitoh T., Satoh T., Kawai T., Ishii J. K., Akira S., Okamoto T., and Matsuura Y.
  • Journal Title: Journal of Virology Volume: 88 Issue: 4 Pages: 2157-2167
  • DOI: 10.1128/jvi.03055-13
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Sasai M, Yamamoto M
  • Journal Title: Int Rev Immunol Volume: 32(2) Issue: 2 Pages: 116-33
  • DOI: 10.3109/08830185.2013.774391
  • Peer Reviewed
• [Journal Article] CREBH determines the severity of sulpyrine- induced fatal shock. (2013)
  • Author(s): Kamiyama, N, et al
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e55800-e55800
  • DOI: 10.1371/journal.pone.0055800
  • Peer Reviewed
• [Journal Article] Noncanonical autophagy is required for type I interferon secretion in response to DNA-immune complexes. (2012)
  • Author(s): Henault J, Martinez J, Riggs JM, Tian J, Mehta P, Clarke L, Sasai M, Latz E, Brinkmann MM, Iwasaki A, Coyle AJ, Kolbeck R, Green DR, Sanjuan MA.
  • Journal Title: Immunity Volume: 37 Pages: 986-97
  • Peer Reviewed
• [Journal Article] A cluster of interferon-γ-inducible p65 GTPases plays a critical role in host defense against Toxoplasma gondii. (2012)
  • Author(s): Yamamoto M, Okuyama M, Ma JS, Kimura T, Kamiyama N, Saiga H, Ohshima J, Sasai M, Kayama H, Okamoto T, Huang DC, Soldati-Favre D, Horie K, Takeda J, Takeda K.
  • Journal Title: Immunity Volume: 37 Pages: 302-13
  • Peer Reviewed
• [Journal Article] Adaptor protein-3 in dendritic cells facilitates phagosomal toll-like receptor signaling and antigen presentation to CD4(+) T cells. (2012)
  • Author(s): Mantegazza AR, Guttentag SH, El-Benna J, Sasai M, Iwasaki A, Shen H, Laufer TM, Marks MS.
  • Journal Title: Immunity Volume: 36 Pages: 782-94
  • Peer Reviewed
• [Presentation] IFN-γ依存性抗トキソプラズマ応答におけるオートファジー蛋白質の役割 (2014)
  • Author(s): 山本雅裕,大嶋淳,馬知秀,笹井美和,李英愛
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛
  • Year and Date: 2014-03-27
• [Presentation] IFN-g依存性抗トキソプラズマ応答におけるオートファジー蛋白質の役割 (2014)
  • Author(s): 山本雅裕、大嶋淳、馬知秀、笹井美和、李英愛
  • Organizer: 第83回に本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphilic dense protein GRA6 (2013)
  • Author(s): JiSu Ma, Miwa Sasai, Kiyoshi Takeda, Masahiro Yamamoto
  • Organizer: JSI 2013
  • Place of Presentation: 幕張メッセ
• [Presentation] インターフェロンγ依存性の細胞内侵入病原体排除におけるオートファジー感染タンパク質の役割の解明 (2013)
  • Author(s): 大嶋淳,笹井美和,山本雅裕,林美加子
  • Organizer: 第138回日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡
• [Presentation] Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense protein GRA6 (2013)
  • Author(s): JiSu Ma, Miwa Sasai, Kiyoshi Takeda, Masahiro Yamamoto
  • Organizer: Annual Meeting of the Japanese Society for Immunology 2013
  • Place of Presentation: MAKUHARI MESSE
• [Presentation] インターフェロンγ依存性の細胞内侵入病原体排除におけるオートファ ジ ー関連タンパク質の役割の解明 (2013)
  • Author(s): 大嶋 淳 , 笹井美和, 山本雅裕, 林 美加子
  • Organizer: 第138回 日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡