| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
In adult bone marrow, it is believed that the specialized microenvironments regulate self-renewal or differentiation of hematopoietic stem/progenitor cells (HSPC). However, our knowledge has been limited especially about the significant species differences in hematopoiesis between mice and humans.
In this study, we found extracellular matrix protein, decorin, which is up-regulated by Wnt3a in mesenchymal stem cells, could maintain stemness phenotype of human HSC. Furthermore, we studied bone-associated cells in the trabecular areas of human marrow, released from femoral heads after collagenase treatment. We found there were two distinct populations according to CD34 and ESAM expression levels in CD34+CD38- cells. CD31+ Flk-1+ endothelial cells were generated after a 5 week long-term culture from CD34hi ESAMhi subset, indicating the unknown cells might be endothelial stem/progenitors.
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