| Project/Area Number |
24890117
|
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| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ophthalmology
|
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| Research Institution | Osaka University |
|---|
Principal Investigator |
|
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| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | アペリン / APJ / 血管新生 / グリア細胞 |
|---|
| Research Abstract |
Using FITC dextran and anti-PECAM-1 antibody in flatmount immunohistochemistry, we checked the role of apelin/APJ signal for barrier fuction in retinal vessels. After injection of FITC dextran fromheart, leakage of dye from developing retinal vessel was observed in APJ knockout (APJ KO) mice compared to wildtype at postnatal day 5. In laser-induced choroidal neovascularization (CNV) model, the size of CNV lesion was significantly decreased in APJ KO mice. Otherwise, an immature glial cell, namely, PDGFRa positive and GFAP negative cell was not found in both wild-type and APJ KO mice. These data suggests that the formation of CNV might be involved in immature glial cells-independent mechanism.
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