Analysis of the role of genes involved in biofilm formation of Porphyromonas gingivalis

• Project/Area Number: 24890118
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: YAMAMOTO Reiko 大阪大学, 歯学研究科(研究院), 特任研究員 (70636133)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯学 / バイオフィルム / Porohyromonas gingivalis / 遺伝子 / 糖 / Porphyromonas gingivalis / ポルフィロモナス ジンジバリス

Research Abstract

The extracellular matrix of the biofilm comprised of protein, carbohydrate and nucleic acid has been shown to functions such as protector of the microbe against antimicrobial agents and immune cells, and stabilizer of the biofilm structure. Therefore, traditional antimicrobial agents have less effect on suppression of biofilm infection, and the development of a novel strategy that targets extracellular matrix instead of microbial cells is necessary for control of the biofilm.
In this study, I focused on an transcription factor PGN_0088 of Porphyromonas gingivalis, that is one of the main pathogen of periodontal disease, and examined how PGN_0088 works on the extracellular matrix in biofilm.
As a result, it became clear that PGN_0088 effects on carbohydrate synthesis in the extracellular matrix of P. gingivalis biofilm.

Research Products

• [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms. (2014)
  • Author(s): Asahi Y, Noiri Y, Miura J, Maezono H, Yamaguchi M, Yamamoto R, Azakami H, Hayashi M, Ebisu S.
  • Journal Title: J Appl Microbiol Volume: 116 Pages: 1164-1171
  • Peer Reviewed
• [Journal Article] Erythritol alters microstructure and metabolomic profiles of biofilm composed of Streptococcus gordonii and Porphyromonas gingivalis (2013)
  • Author(s): Hashino E, Kuboniwa M, Alghamdi SA, Yamaguchi M, Yamamoto R, Cho H, Amano A
  • Journal Title: Mol Oral Microbiol Volume: 28 Pages: 435-451
• [Journal Article] Porphyromonas gingivalis biofilms persist after chlorhexidine treatment (2013)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Yamamoto R, Asahi Y, Maezono H, Hayashi M, Ebisu S
  • Journal Title: Eur J Oral Sci Volume: 121 Pages: 162-168
• [Journal Article] The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms (2013)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuboniwa M, Hayashi M, Ebisu S
  • Journal Title: PLoS One Volume: 8
• [Journal Article] Erythritol alters microstructure and metabolomic profiles of biofilm composed of Streptococcus gordonii and Porphyromonas gingivalis. (2013)
  • Author(s): Hashino E, Kuboniwa M, Alghamdi SA, Yamaguchi M, Yamamoto R, Cho H, Amano A.
  • Journal Title: Mol Oral Microbiol Volume: 28 Pages: 435-451
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis biofilms persist after chlorhexidine treatment. (2013)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Yamamoto R, Asahi Y, Maezono H, Hayashi M, Ebisu S.
  • Journal Title: Eur J Oral Sci Volume: 121 Pages: 162-168
  • Peer Reviewed
• [Journal Article] The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porohyromonas gingivalis ATCC 33277 biofilms. (2013)
  • Author(s): Yamamoto R
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis の PGN_0088 遺伝子の役割解析 - バイオフィルム形成とマクロライド系抗生物質の抗バイオフィルム作用について - (2013)
  • Author(s): 山本れいこ
  • Journal Title: Bacterial Adherence & Biofilm Volume: 26 Pages: 65-71
• [Journal Article] Porphyromonas gingivalisのPGN_0088遺伝子の役割解析–バイオフィルム形成とマクロライド系抗生物質の抗バイオフィルム作用について- (2012)
  • Author(s): 山本れいこ,野杁由一郎,山口幹代,朝日陽子,前薗葉月,呉本勝隆,林美加子,恵比須繁之
  • Journal Title: Bacterial adherence & Biofilm Volume: 26 Pages: 65-71
• [Journal Article] Bone Marrow-Derived HipOP Cell Population Is Markedly Enriched in Osteoprogenitors (2012)
  • Author(s): Itoh S, Matsushita K, Ikeda S, Yamamoto Y, Yamauchi Y, Yoshioka S, Yamamoto R, Ebisu S, Hayashi M, Aubin JE
  • Journal Title: Int J Mol Sci Volume: 13 Pages: 10229-10235
• [Presentation] Porphyromonas gingivalisのPGN_0088遺伝子はバイオフィルム形成に関与する (2013)
  • Author(s): 山本れいこ,野杁由一郎,山口幹代,朝日陽子,前薗葉月,呉本勝隆,林美加子,恵比須繁之
  • Organizer: 日本歯科保存学会2013年度秋期学術大会(第139回)
  • Place of Presentation: 秋田市
• [Presentation] Role of PGN_0088 of Porphyromonas gingivalis biofilm formation (2013)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuboniwa M, Hayashi M, Ebisu S
  • Organizer: 2^<nd> IADR-APR 2013、Bangkok
  • Place of Presentation: Thailand
• [Presentation] Role of PGN_0088 of Porphyromonas gingivalis biofilm formation (2013)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, MaezonoH, Kuboniwa M, Hayashi M, Ebisu S.
  • Organizer: 2nd IADR-APR 2013
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Porphyromonas gingivalisのPGN_0088遺伝子はバイオフィルム形成に関与する (2013)
  • Author(s): 山本れいこ, 野杁 由一郎, 山口 幹代, 朝日 陽子, 前薗 葉月, 呉本 勝隆, 林 美加子, 恵比須 繁之
  • Organizer: 日本歯科保存学会2013年度秋季学術大会(第139回)
  • Place of Presentation: 秋田市
• [Presentation] 根尖孔外バイオフィルムモデルにおけるラット根尖病巣のマイクロCT解析 (2012)
  • Author(s): 呉本勝隆,野杁由一郎,石本卓也,永山智崇,騎馬和歌子,前薗葉月,山本れいこ,米田直道,中野貴由,林美加子,恵比須繁之
  • Organizer: 日本歯科保存学会2012年度秋期学術大会(第137回)
  • Place of Presentation: 広島市
• [Presentation] Role of PGN_0088 of Porphyromonas gingivalis–Biofilm Formation and Antibiofilm Effect of Macrolide Antibiotics - (2012)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuremoto K, Hayashi M and Ebisu S
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, USA
• [Presentation] Dispersal of Porphyromonas gingivalis Biofilm Induced by Environmental Factors and Proteases (2012)
  • Author(s): Yamaguchi M, Noiri Y, Asahi Y, Maezono H, Yamamoto R, Kuremoto K, Hayashi M, Ebisu S
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, USA
• [Presentation] Altered Protein Expressions by Sub - Minimum Inhibitory Concentrations of Azithromycin on Porphyromonas gingivalis Biofilms (2012)
  • Author(s): Maezono H, Noiri Y, Asahi Y, Yamaguchi M, Yamamoto R, Kuremoto K, Hayashi M, Ebisu S
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, USA
• [Presentation] Porphyromonas gingivalisのPGN_0088遺伝子の役割解析–バイオフィルム形成とマクロライド系抗生物質の抗バイオフィルム作用について- (2012)
  • Author(s): 山本れいこ,野杁由一郎,山口幹代,朝日陽子,前薗葉月,呉本勝隆,林美加子,恵比須繁之
  • Organizer: 第26回Bacterial Adherence & Biofilm学術集会
  • Place of Presentation: 大阪市
• [Presentation] 難治性根尖性歯周炎に関わるバイオフィルム構成細菌種と臨床症状との関係 (2012)
  • Author(s): 薮根敏晃,野杁由一郎,山本れいこ,山口幹代,朝日陽子,前薗葉月,永山智崇,呉本勝隆,騎馬和歌子,林美加子,恵比須繁之
  • Organizer: 日本歯科保存学会2012年春季学術大会(第136回)
  • Place of Presentation: 宜野湾市
• [Presentation] Porphyromonas gingivalisのバイオフィルム形成におけるプロテアーゼの役割 (2012)
  • Author(s): 山口幹代,野杁由一郎,朝日陽子,前薗葉月,山本れいこ,呉本勝隆,林美加子,恵比須繁之
  • Organizer: 日本歯科保存学会2012年春季学術大会(第136回)
  • Place of Presentation: 宜野湾市
• [Presentation] Role of PGN_0088 of Porphyromonas gingivalis - Biofilm Formation and Antibiofilm Effect of Macrolide Antibiotics - (2012)
  • Author(s): Yamamoto R
  • Organizer: 6th ASM Conference on Biofilms（学会開期; 2012年 9月 29日-10月 4日）
  • Place of Presentation: Miami, USA