Clarification of epigenetic regulation for osseointegration

• Project/Area Number: 24890122
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Osaka University
• Principal Investigator: TAKASHIMA Rikako 大阪大学, 歯学部附属病院, 医員 (00632118)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エピジェネティック / オッセオインテグレーション

Research Abstract

I tried to clarify the epigenetic regulation of osseointegration.Iexamined the osteoblast differentiation of osteoblast-like cell with titanium under the overexpression of BMP2.The result was that titanium with overexpression of BMP2 produced more increase of type I collagen,osteocalcin and alkaline phosphatase expression compared to titanium or BMP2 independently.Next,I investigated the impact of smoking on osseointegration.I did the same experiment by adding nicotine.Then,the expression level of type I collagen,osteocalcin and alkaline phosphatase were decreased.Furthermore I made a study of histone modification by Chip assay.I observed H3K9 of type I collagen promoter was more methylated in MC3T3-E1 cell with titanium and BMP2 than BMP2 independently.