Study for the development of new fracture treatment by the adhesive technology evolution and molecular engineering

• Project/Area Number: 24890131
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: 独立行政法人国立病院機構福山医療センター(臨床研究部) (2013); Okayama University (2012)
• Principal Investigator: SHIOZAKI Yasuyuki 独立行政法人国立病院機構福山医療センター(臨床研究部), その他部局等, その他 (00596041)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 成長因子 / 骨形成 / 進化分子工学

Research Abstract

BMP family proteins are widely used for biological research and medical application because of their ability to induce bone morphogenesis at adult homeostasis and differentiation of epidermis and dorsal neural tube at early development. Utilizing growth factors that stimulate bone morphogenesis with a bone implant is a promising approach in regenerative medicine. We create a fusion protein consisting of bone morphologic protein-4 and a collagen-binding domain polypeptide of fibronectin. This fusion protein (CBD-BMP4), produced by a baculovirus expression system, exhibited much strong collagen binding activity. The aim of this study is to examine the effect of this fusion protein for repair of bone defect.

Research Products

• [Journal Article] A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering (2014)
  • Author(s): Tetsuro Mazaki, Yasuyuki Shiozaki, Kentaro Yamane, Aki Yoshida, Mariko Nakamura, Yasuhiro Yoshida, Di Zhou, Takashi Kitajima, Masato Tanaka, Yoshihiro ito, Toshifumi Ozaki, and Akihiro Matsukawa
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 4457-4457
  • DOI: 10.1038/srep04457
  • NAID: 120005526619
  • Peer Reviewed
• [Journal Article] Enhanced in vivo osteogenesis by nanocarrier-fused BMP4 (2013)
  • Author(s): Y. Shiozaki
  • Journal Title: Int. J. Nanomed. Volume: 8 Pages: 1349-1360
  • DOI: 10.2147/ijn.s44124
  • Peer Reviewed
• [Presentation] (2013)
  • Organizer: 第86回日本整形外科学会学術総会
  • Place of Presentation: 広島グリーンアリーナ
• [Presentation] 遺伝子改変 BMP collagen binding domain BMP を用いたウサギ大腿骨での骨形成 (2013)
  • Author(s): 塩崎泰之
  • Organizer: 第86回日本整形外科学会学術総会
  • Place of Presentation: 広島グリーンアリーナ
• [Presentation] (2012)
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] (2012)
  • Organizer: 日本バイオマテリアル学会シンポジウム
  • Place of Presentation: 仙台国際センター
• [Presentation] マウスを用いた遺伝子改変成長因子 Collagen binding domain BMP4 の効果 (2012)
  • Author(s): 塩崎泰之
  • Organizer: 日本バイオマテリアル学会大会シンポジウム2012
  • Place of Presentation: 仙台国際センター