Functional analysis of candidate pseudopodial molecules of breast cancer identified by a novel application of laser proteomics

• Project/Area Number: 24890137
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Hiroshima University
• Principal Investigator: MIMAE TAKAHIRO 広島大学, 原爆放射線医科学研究所, 助教 (00634081)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 癌浸潤 / プロテオミクス

Research Abstract

The cancer invasion has a potential to threaten the lives of patients. Therefore, the regulation of invasion will lead to conquest of cancer. In the present study, we attempted to disclose the mechanism of invadopodia or pseudopodia microprocesses formation which are known to be involved in cell motility or cancer cell invasion. We developed a novel application for precise isolation of invadopodia or pseudopodia. The protein fraction of pseudopodia was collected by this novel application, then, the candidate pseudopodial molecules were identified. In addition, the functional analysis of some candidate molecules was performed. The outcomes of the study was published in papers and annual meeting of AACR2013. The present study will provide further understandings of cancer research.

Research Products

• [Journal Article] α-Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma (2014)
  • Author(s): Masaoki Ito, Man Hagiyama, Takahiro Mimae, Takao Inoue, Takashi Kato, Azusa Yoneshige, Jun Nakanishi, Tadashi Kondo, Morihito Okada, Akihiko Ito
  • Journal Title: Breast Cancer Res Treat Volume: 144(1) Pages: 59-69
  • Peer Reviewed
• [Journal Article] α- Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma. (2014)
  • Author(s): Ito M, Hagiyama M, Mimae T, Inoue T, Kato T, Yoneshige A, Nakanishi J, Kondo T, Okada M, Ito A.
  • Journal Title: Breast Cancer Res Treat Volume: 144 Issue: 1 Pages: 59-69
  • DOI: 10.1007/s10549-014-2859-0
  • Peer Reviewed / Open Access
• [Journal Article] Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis. (2012)
  • Author(s): Ito A, Murakami Y, Kondo T et al.
  • Journal Title: Lab Invest Volume: 92 Issue: 9 Pages: 1374-1385
  • DOI: 10.1038/labinvest.2012.98
  • Peer Reviewed
• [Presentation] Novel application for pseudopodial proteomics using excimer laserablation and two-dimensional difference gel electrophoresis (2013)
  • Author(s): Takahiro Mimae, Akihiko Ito, Man Hagiyama, Tadashi Kondo, Morihito Okada
  • Organizer: AACR Annual Meeting 2013
  • Place of Presentation: Washington D.C.
  • Year and Date: 2013-04-09
• [Presentation] Novel application for pseudopodial proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis (2013)
  • Author(s): Takahiro Mimae, Akihiko Ito, Man Hagiyama, Tadashi Kondo, Morihito Okada
  • Organizer: AACR
  • Place of Presentation: Washington D.C. (USA)