Elucidation of of non-alcoholic steatohepatitis exacerbation mechanism by dental infection and effects of dental treatment

• Project/Area Number: 24890138
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Hiroshima University
• Principal Investigator: FURUSHO Hisako 広島大学, 医歯薬保健学研究院(歯), 助教 (00634461)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: P.gingivalis / 歯性感染 / NASH / 免疫 / 歯科処置 / LPS / E.coli / パルミチン酸 / 肝構成細胞 / 炎症性サイトカイン

Research Abstract

Effect of Porphyromonas gingivalis (P.g.) on non-alcoholic steatohepatitis (NASH) were examined. Various bacterial components (lipopolysaccharaide;LPS, flagellin and DNA) activate natural immune pathways through TLR2, 4, 5 and 9, resulting in upregulation of inflammatory cytokine expression and inflammasome activation . In fatty liver, showing upreglation TLR2 expression, the increased sensitivity of P.g.-LPS, which is TLR2 ligand, may cause stronger pathogenesis. Moreover, elimination of P.g.-odontogenic infection in P.g.-infected / high fat diet induced NASH mouse model reduced inflammatory cytokines expression and inflammatory cytokines expression and inflammatory cytokines expression and inflammation in liver. It indicates that dental therapy may have beneficial impact on management of NASH.

Research Products

• [Journal Article] Dental infection of Porphyromonas gingivalis exacerbates high fat diet-induced steatohepatitis in mice (2013)
  • Author(s): FurushoH,Miyauchi M,Hyogo H,Inubushi T,Ao M,Ouhara K,Hisatune J,Kurihara H,Sugai M,Hayes CN,Nakahara T,Akikata H,Takahashi S,Chayama K,Takata T
  • Journal Title: J Gastroenterol Volume: 48(11) Issue: 11 Pages: 1259-70
  • DOI: 10.1007/s00535-012-0738-1
  • Peer Reviewed
• [Journal Article] Dental infection of Porphyromonas gingivalis exacerbates high fat diet-induced steatohepatitis in mice (2013)
  • Author(s): Hisako Furusho
  • Journal Title: Journal of Gastroenterology Volume: 48 Pages: 1259-1270
  • Peer Reviewed
• [Presentation] Effect of Porphyromonas gingivalis on the progression non-alcoholic steatohepatitis (2013)
  • Author(s): FurushoH,Miyauchi M,Ao M,Inubushi T,Takata T
  • Organizer: International Association of Dental Research General Session
  • Place of Presentation: Seattle
• [Presentation] Effect of Porphyromonas gingivalis on the progression non-alcoholic steatohepatitis. (2013)
  • Author(s): Hisako Furusho
  • Organizer: 国際歯科学研究会（IADR)
  • Place of Presentation: シアトル（アメリカ合衆国）