| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Human ESCs and iPSCs are commonly maintained on mouse feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture media containing undefined or unknown components has limited the development of applications for pluripotent cells. Therefore we developed a serum-free medium hESF9. We have successfully generated hiPSCs with retroviral vectors or sendai virus vectors in serum- and feeder-free defined medium on fibronectin. As a result we successfully generated hiPSCs using human dental pulp cells from cleidocranial dysplasia (CCD), moreover the cells retained pluripotency.
As this simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions, and can eliminate the risk might be brought by undefined pathogens.
In addition, CCD-iPSCs would be beneficial to clarify the molecular mechanism involved in the disease.
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