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Generation of disease-specific human induced pluripotent stem (iPS) cells of a patient with Cleidocranial dysplasia in serum- and feeder-free defined culture.

Research Project

Project/Area Number 24890139
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionHiroshima University

Principal Investigator

YAMASAKI Sachiko  広島大学, 大学病院, 歯科診療医 (00632001)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsiPS細胞 / 再生医療 / 人工多能性幹細胞 / 再生歯学 / 遺伝子疾患 / 細胞培養
Research Abstract

Human ESCs and iPSCs are commonly maintained on mouse feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture media containing undefined or unknown components has limited the development of applications for pluripotent cells. Therefore we developed a serum-free medium hESF9. We have successfully generated hiPSCs with retroviral vectors or sendai virus vectors in serum- and feeder-free defined medium on fibronectin. As a result we successfully generated hiPSCs using human dental pulp cells from cleidocranial dysplasia (CCD), moreover the cells retained pluripotency.
As this simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions, and can eliminate the risk might be brought by undefined pathogens.
In addition, CCD-iPSCs would be beneficial to clarify the molecular mechanism involved in the disease.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (42 results)

All 2014 2013 2012 Other

All Journal Article (21 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (21 results)

  • [Journal Article] Generation of Human induced pluripotent Stem (iPS) cells in serum- and feeder-free defined culture and TGF-β1 regulation of pluripotency2014

    • Author(s)
      Yamasaki S, Taguchi Y, Shimamoto A, Mukasa H, Tahara H, Okamoto T.
    • Journal Title

      PLoS One

      Volume: 9(1) Issue: 1 Pages: 1-13

    • DOI

      10.1371/journal.pone.0087151

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term serial cultivation of mouse induced pluripotent stem cells in serum-free and feeder-free defined medium2013

    • Author(s)
      Yamasaki S, Nabeshima K, Sotomaru Y, Taguchi Y, Mukasa H, Furue MK, Sato JD, Okamoto T
    • Journal Title

      Int J Dev Biol

      Volume: 57(9-10) Issue: 9-10 Pages: 715-724

    • DOI

      10.1387/ijdb.130173to

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 多科連携により初期治療と欠損部の再建を行った重症口腔顎顔面外傷の一例2013

    • Author(s)
      吉岡幸男、虎谷茂昭、伊藤翼、山崎佐知子、藤井良典、小泉浩一、林堂安貴、岡本哲治
    • Journal Title

      口腔顎顔面外傷

      Volume: 第12巻 Pages: 37-43

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum- and feeder-free culture2013

    • Author(s)
      H Mukasa, S Yamasaki, Y Taguchi, A Shimamoto, H Tahara, T Okamoto
    • Journal Title

      In Vitro Cellular & Developmental Biology-Animal

      Volume: Vol. 49 Pages: 44-45

    • Related Report
      2013 Final Research Report
  • [Journal Article] Generation and serial cultivation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free defined culture2013

    • Author(s)
      Y Taguchi, S Yamasaki, H Mukasa, A Shimamoto, H Tahara, T Okamoto
    • Journal Title

      In Vitro Cellular & Developmental Biology-Animal

      Volume: Vol.49 Pages: 45-45

    • Related Report
      2013 Final Research Report
  • [Journal Article] 頬部腫脹を契機に診断されたネフローゼ症候群を伴った木村病の1例2013

    • Author(s)
      神田拓、虎谷茂昭、山崎佐知子、伊藤奈七子、小川郁子、岡本哲治
    • Journal Title

      口腔外科学会誌

      Volume: 第59巻第2号 Pages: 83-87

    • NAID

      10031156083

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞(iPS細胞)の樹立2013

    • Author(s)
      向笠英恵、山崎佐知子、田口有紀、嶋本顕、田原栄俊、岡本哲治
    • Journal Title

      日本口腔組織培養学会誌

      Volume: 第22巻第1号 Pages: 15-16

    • Related Report
      2013 Final Research Report
  • [Journal Article] Long-term serial cultivation of mouse induced pluripotent stem cells in serum-free and feeder-free defined medium2013

    • Author(s)
      S YAMASAKI, K NABESHIMA, Y SOTOMARU, M K FURUE, J D SATO, T OKAMOTO
    • Journal Title

      Int. J. Dev. Biol.

      Volume: 57(9-10) Pages: 715-724

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 多科連携により初期治療と欠損部の再建を行った重症口腔顎顔面外傷の一例2013

    • Author(s)
      吉岡幸男、虎谷茂昭、伊藤翼、山崎佐知子、藤井良典、小泉浩一、久保隆靖、林堂安貴、岡本哲治
    • Journal Title

      口腔顎顔面外傷

      Volume: 12 Pages: 37-43

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum- and feeder-free culture.2013

    • Author(s)
      H Mukasa, S Yamasaki, Y Taguchi, A Simamoto, H Tahara, T Okamoto.
    • Journal Title

      In Vitro Cell. Dev. Biol. Anim.

      Volume: 49 Pages: 44-45

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Generation and serial cultivation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free defined culture.2013

    • Author(s)
      Y Taguchi, S Yamasaki, H Mukasa, A Simamoto, H Tahara, T Okamoto.
    • Journal Title

      In Vitro Cell. Dev. Biol. Anim.

      Volume: 49 Pages: 45-45

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 頬部腫脹を契機に診断されたネフローゼ症候群を伴った木村病の1例2013

    • Author(s)
      神田拓、虎谷茂昭、山崎佐知子、岡本哲治 ら
    • Journal Title

      口腔外科学会誌

      Volume: 59(2) Pages: 83-87

    • NAID

      10031156083

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞( iPS細胞)の樹立2013

    • Author(s)
      山崎佐知子、嶋本顕、田原栄俊、岡本哲治
    • Journal Title

      日本口腔科学会雑誌

      Volume: 62(1) Pages: 153-153

    • Related Report
      2012 Annual Research Report
  • [Journal Article] ヒト人工多能性幹細胞 (iPS細胞)の単層無血清培養系の確立2013

    • Author(s)
      山崎佐知子、田口有紀、嶋本顕、田原栄俊、岡本哲治
    • Journal Title

      日本口腔科学会雑誌

      Volume: 62(1) Pages: 153-153

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞 (iPS細胞)の樹立2013

    • Author(s)
      向笠英恵、山崎佐知子、田口有紀、嶋本顕、田原栄俊、岡本哲治
    • Journal Title

      日本口腔組織培養学会

      Volume: 22 Pages: 15-16

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Ectomesenchymal chondromyxoid tumor of the tongue : Insights on histogenesis2012

    • Author(s)
      Y Yukio, I OGAWA, T Tsunematsu, T SAKAUE, S YAMASAKI, Y FUKUI, Y HAYASHIDO, S TORATANI, T OKAMOTO
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol

      Volume: 115(2) Pages: 223-240

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of human induced pluripotent stem (iPS) cells in serum- and feeder-free defined culture from fetal lung fibroblasts and dental pulp2012

    • Author(s)
      YAMASAKI S, SHIMAMOTO A, TAHARA H, OKAMOTO T
    • Journal Title

      In Vitro Cellular & Developmental Biology-Animal

      Volume: Volume 48 Pages: 48-49

    • Related Report
      2013 Final Research Report
  • [Journal Article] 筋上皮癌との鑑別を要したectomesenchymal chondromyxoid tumorの1例2012

    • Author(s)
      小川郁子・常松貴明・安藤俊範・大林真理子・山崎佐知子・高田隆
    • Journal Title

      日本唾液腺学会誌

      Volume: 第53巻 Pages: 40-40

    • Related Report
      2013 Final Research Report
  • [Journal Article] Ectomesenchymal chondromyxoid tumor of the tongue: Insights on  histogenesis.2012

    • Author(s)
      Yoshioka Y, Ogawa I, Yamasaki S, Okamoto T. et al.
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol

      Volume: 115(2) Pages: 233-240

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of human induced pluripotent stem (iPS) cells in serum- and feeder-free defined culture from fetal lung fibroblasts and dental pulp cells derived from a patient with cleidocranial dysplasia.2012

    • Author(s)
      YAMASAKI S, SHIMAMOTO A, TAHARA H and OKAMOTO T.
    • Journal Title

      In Vitro Cellular & Developmental Biology-Animal

      Volume: 48 Pages: 48-49

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 筋上皮癌との鑑別を要したectomesenchymal chondromyxoid tumor の1 例2012

    • Author(s)
      小川郁子・常松貴明・安藤俊範・大林真理子・山崎佐知子・高田 隆
    • Journal Title

      日本唾液腺学会誌

      Volume: 53 Pages: 40-40

    • Related Report
      2012 Annual Research Report
  • [Presentation] センダイウイルスを用いた無血清培養系におけるhiPS細胞の樹立と維持2013

    • Author(s)
      濱田充子、山崎佐知子、赤木恵理、大高真奈美、西村健、中西真人、岡本哲治
    • Organizer
      第50回日本口腔組織培養学会総会
    • Place of Presentation
      日本歯科大学生命歯学部九段ホール、東京
    • Year and Date
      2013-11-24
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Generation of human induced pluripotent stem (iPS) cells in serum- and feeder-free defined culture from dental pulp cells2013

    • Author(s)
      S YAMASAKI,T OKAMOTO
    • Organizer
      5th Hiroshima conference on Education and Science in Dentistry
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2013-10-12
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum- and feeder-free culture2013

    • Author(s)
      H Mukasa, S Yamasaki, Y Taguchi, A Shimamoto, H Tahara, T Okamoto
    • Organizer
      2013 In Vitro Biology Meeting
    • Place of Presentation
      Providence, Rhode Island, USA
    • Year and Date
      2013-06-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation and serial cultivation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free defined culture2013

    • Author(s)
      Y Taguchi, S Yamasaki, H Mukasa, A Shimamoto, H Tahara, T Okamot
    • Organizer
      2013 In Vitro Biology Meeting
    • Place of Presentation
      Providence, Rhode Island, USA
    • Year and Date
      2013-06-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 単層無血清培養系での歯髄由来細胞を用いたヒト人工多能性幹細胞(iPS細胞)の樹立および維持2013

    • Author(s)
      田口有紀,山崎佐知子,嶋本顕,向笠英恵,田原栄俊、岡本哲治
    • Organizer
      第67回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      栃木県総合文化センター、栃木
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞(iPS細胞)の樹立2013

    • Author(s)
      向笠英恵,山崎佐知子,田口有紀,嶋本顕,田原栄俊,岡本哲治
    • Organizer
      第67回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      栃木県総合文化センター、栃木
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum-and feeder-free culture2013

    • Author(s)
      Mukasa H, Yamasaki S, Okamoto T
    • Organizer
      The 2th International Symposium Suggestion for the Renaissance from Radiation Disaster
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2013-02-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free culture condition2013

    • Author(s)
      TAGUCHI Y, YAMASAKI S, SHIMAMOTO A, TAHARA H, OKAMOTO T
    • Organizer
      The 2th International Symposium Suggestion for the Renaissance from Radiation Disaster
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2013-02-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum- and feeder-free culture.2013

    • Author(s)
      H Mukasa, S Yamasaki, Y Taguchi, A Simamoto, H Tahara, T Okamoto.
    • Organizer
      2013 In Vitro Biology Meeting
    • Place of Presentation
      Providence, Rhode Island, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Generation and serial cultivation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free defined culture.2013

    • Author(s)
      Y Taguchi, S Yamasaki, H Mukasa, A Simamoto, H Tahara, T Okamoto.
    • Organizer
      2013 In Vitro Biology Meeting
    • Place of Presentation
      Providence, Rhode Island, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 単層無血清培養系での歯髄由来細胞を用いたヒト人工多能性幹細胞 (iPS細胞)の樹立および維持2013

    • Author(s)
      田口有紀 山崎佐知子 嶋本顕 向笠英恵 田原栄俊 岡本哲治
    • Organizer
      第67回NPO法人日本口腔科学会学術集会総会
    • Place of Presentation
      栃木県総合文化センター、栃木
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞 (iPS細胞)の樹立2013

    • Author(s)
      向笠英恵 山崎佐知子 田口有紀 嶋本顕 田原栄俊 岡本哲治
    • Organizer
      第67回NPO法人日本口腔科学会学術集会総会
    • Place of Presentation
      栃木県総合文化センター、栃木
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋上皮癌との鑑別を要したectomesenchymal chondromyxoid tumorの1例2012

    • Author(s)
      小川郁子・常松貴明・安藤俊範・大林真理子・山崎佐知子・高田隆
    • Organizer
      第57回日本唾液腺学会学術大会
    • Place of Presentation
      文京学院大学本郷キャンパス
    • Year and Date
      2012-12-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞(iPS細胞)の樹立2012

    • Author(s)
      向笠英恵、山崎佐知子、田口有紀、嶋本顕、田原栄俊、岡本哲治
    • Organizer
      第49回日本口腔組織培養学会
    • Place of Presentation
      広島大学広仁会館
    • Year and Date
      2012-11-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of human induced pluripotent stem (iPS) cells in serum- and feeder-free defined culture from fetal lung fibroblasts and dental pulp cells derived from a patient with cleidocranial dysplasia2012

    • Author(s)
      YAMASAKI S, SHIMAMOTO A, TAHARA H, OKAMOTO T
    • Organizer
      2012 World congress on In Vitro Biology
    • Place of Presentation
      Bellevue, Washington, USA
    • Year and Date
      2012-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞(iPS細胞)の樹立2012

    • Author(s)
      山崎佐知子、嶋本顕、田原栄俊、岡本哲治
    • Organizer
      第66回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト人工多能性幹細胞(iPS細胞)の単層無血清培養系の確立2012

    • Author(s)
      田口有紀、山崎佐知子、嶋本顕、田原栄俊、岡本哲治.
    • Organizer
      第66回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者歯髄由来細胞を用いた疾患特異的ヒト人工多能性幹細胞 (iPS細胞)の樹立

    • Author(s)
      向笠英恵、山崎佐知子、田口有紀、嶋本顕、田原栄俊、岡本哲治
    • Organizer
      第49回日本口腔組織培養学会
    • Place of Presentation
      広島大学広仁会館
    • Related Report
      2012 Annual Research Report
  • [Presentation] 筋上皮癌との鑑別を要したectomesenchymal chondromyxoid tumor の1 例

    • Author(s)
      小川郁子・常松貴明・安藤俊範・大林真理子・山崎佐知子・高田 隆
    • Organizer
      第57回日本唾液腺学会学術大会
    • Place of Presentation
      文京学院大学本郷キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum-and feeder-free culture.

    • Author(s)
      Mukasa H, Yamasaki S, Okamoto T, et al.
    • Organizer
      The 2th International Symposium Suggestion for the Renaissance from Radiation Disaster
    • Place of Presentation
      広島国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Generation of induced pluripotent stem cells from dental pulp cells in serum-free and feeder-free culture condition.

    • Author(s)
      TAGUCHI Y, YAMASAKI S, SHIMAMOTO A, TAHARA H, OKAMOTO T
    • Organizer
      The 2th International Symposium Suggestion for the Renaissance from Radiation Disaster
    • Place of Presentation
      広島国際会議場
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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