The induction of immune tolerance and the improvement islet transplantation with histone deacetylase inhibitors.
Project/Area Number |
24890149
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Digestive surgery
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Research Institution | The University of Tokushima |
Principal Investigator |
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Project Period (FY) |
2012-08-31 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 免疫寛容誘導 / ドナー特異的輸血 / ヒストン脱アセチル化酵素阻害剤 |
Research Abstract |
We focused on histone deacetylase inhibitors (HDACi) because it was reported that inhibition of HDAC activity prevented Treg differentiation into IL17-producing cells. We therefore sought to enhance Treg while suppressing Th17 cells using DST with HDACi to prolong graft survival. To stimulate Treg by DST, we used donor splenocytes. In DST with HDACi group, Foxp3 mRNA expression and Treg population increased in the thymus and spleen, whereas Th17 population decreased. qPCR analysis of lymphocyte mRNA indicated that Foxp3, IL-10, and TGF-b expression increased. However, interleukin 17a, Stat3 (Th17), and IFN-g expression decreased in DST + HDACi group, relative to DST alone. Moreover, DST treated with HDACi prolonged graft survival relative to controls in mice islet transplantation. DST with HDACi may therefore have utility in islet transplantation.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Specific miRNA expression profiles of non-tumor liver tissue predict a risk for recurrence of hepatocellular carcinoma.2014
Author(s)
Utsunomiya T, Ishikawa D, Asanoma M, Yamada S, Iwahashi S, Kanamoto M, Arakawa Y, Ikemoto T, Morine Y, Imura S, Ishibashi H, Takasu C, Shimada M.
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Journal Title
Hepatol Res.
Volume: 印刷中
Issue: 6
Pages: 631-638
DOI
Related Report
Peer Reviewed
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[Journal Article] Elevation of high-mobility group box 1 after clinical autologous islet transplantation and its inverse correlation with outcomes2014
Author(s)
Itoh T, Iwahashi S, Kanak MA, Shimoda M, Takita M, Chujo D, Tamura Y, Rahman AM, Chung WY, Onaca N, Coates PT, Dennison AR, Naziruddin B, Levy MF, Matsumoto S
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Journal Title
Cell Transplant
Volume: 23
Issue: 2
Pages: 153-165
DOI
Related Report
Peer Reviewed
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[Journal Article] CXC receptor 4 and stromal cell-derived factor 1 in primary tumors and liver metastases of colorectal cancer.2013
Author(s)
Yamada S, Shimada M, Utsunomiya T, Morine Y, Imura S, Ikemoto T, Mori H, Arakawa Y, Kanamoto M, Iwahashi S, Saito Y.
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Journal Title
Journal of Surgical Research
Volume: 187
Issue: 1
Pages: 107-112
DOI
Related Report
Peer Reviewed
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[Journal Article] Autoimmune chronic pancreatitis with IgG4-related pancreatic pseudocyst in a patient undergoing total pancreatectomy followed by autologous islet transplantation: a case report.2013
Author(s)
Takita M, Itoh T, Matsumoto S, Shimoda M, Chujo D, Iwahashi S, Tamura Y, Onaca N, Naziruddin B, Bartlett BL, Levy MF
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Journal Title
Pancreas
Volume: 42
Issue: 1
Pages: 175-177
DOI
Related Report
Peer Reviewed
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