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ion transport and regulation of the ion transport in the inner ear and endolymphatic sac

Research Project

Project/Area Number 24890155
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionKagawa University

Principal Investigator

MIYASHITA Takenori  香川大学, 医学部, 講師 (60363214)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords内リンパ嚢 / 内耳 / イオン輸送 / イオンチャネル / メニエール病 / イオンイメージング / 内リンパ調節 / 内リンパ水腫 / 内耳生理 / 耳科学 / イメージング
Research Abstract

We demonstrated the expression of Prox1 in all parts of the endolymphatic sac epithelia in adult stage. Prox1 was used as a marker for the endolymphatic sac epithelia. The use of Prox1-GFP mice made possible the whole-mount imaging and three-dimensional observations of the endolymphatic sac epithelial cells through decalcified temporal bone. Using this whole-mount imaging technique and vascular staining with dextran-rhodamine, the endolymphatic sac was observed. In the proximal portion, the endolymphatic sac was narrow and surrounded by few blood vessels. In the intermediate portion, which is the most active ion transporting part, the endolymphatic sac protruded and was surrounded by many branched blood vessels. In the distal portion, the endolymphatic sac was close to the sigmoid sinus. The same samples were sectioned and stained by immunohistochemistry. This whole-mount imaging technique of the endolymphatic sac will be a very useful method for endolymphatic sac research.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (24 results)

All 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Remarks (1 results)

  • [Journal Article] Cystic fibrosis transmembrane conductance regulator in the endolymphatic sac of the rat2014

    • Author(s)
      Matsubara A, Miyashita T, Inamoto R, Hoshikawa H, Mori N
    • Journal Title

      Auris Nasus Larynx

      Volume: - Issue: 5 Pages: 409-412

    • DOI

      10.1016/j.anl.2014.02.005

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization and proliferation of lymphatic vessels in the tympanic membrane in normal state and regeneration2013

    • Author(s)
      Miyashita T, Burford JL, Hong YK, Gevorgyan H, Lam L, Mori N, Peti-Peterdi J
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 440 Issue: 3 Pages: 371-3

    • DOI

      10.1016/j.bbrc.2013.09.054

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Presence of adrenergic receptors in rat endolymphatic sac epithelial cells.2013

    • Author(s)
      Matsubara Ai
    • Journal Title

      Journal of Membrane Biology

      Volume: 246 Issue: 2 Pages: 109-114

    • DOI

      10.1007/s00232-012-9508-5

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ラット内リンパ嚢におけるアドレナリンレセプターの発現2013

    • Author(s)
      松原あい、宮下武憲、稲本隆平、森  望
    • Journal Title

      耳鼻咽喉科ニューロサイエンス

      Volume: 27 Pages: 6-9

    • Related Report
      2013 Annual Research Report
  • [Journal Article] イソプロテレノールの内耳への影響2013

    • Author(s)
      稲本隆平、宮下武憲、森  望
    • Journal Title

      耳鼻咽喉科ニューロサイエンス

      Volume: 27 Pages: 10-14

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 鼓膜のリンパ管局在、鼓膜再生時の反応2013

    • Author(s)
      宮下武憲、James Burford、Young-Kwon Hong、森  望、Janos Peti-Peterdi
    • Journal Title

      耳鼻咽喉科ニューロサイエンス

      Volume: 27 Pages: 48-49

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Presence of FXYD6 in the endolymphatic sac epithelia2012

    • Author(s)
      Miyashita Takenori
    • Journal Title

      Neuroscience Letters

      Volume: 513 Issue: 1 Pages: 47-50

    • DOI

      10.1016/j.neulet.2012.02.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The mRNA of claudins is expressed in the endolymphatic sac epithelia2012

    • Author(s)
      Ai Matsubara, Takenori Miyashita
    • Journal Title

      Auris Nasus Larynx

      Volume: 39 Issue: 4 Pages: 361-364

    • DOI

      10.1016/j.anl.2011.07.004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the vestibular aqueduct in Meniere's disease using multiplanar reconstruction images of CT2012

    • Author(s)
      Takenori Miyashita, Yoshihiro Toyama
    • Journal Title

      Auris Nasus Larynx

      Volume: 39 Issue: 6 Pages: 567-571

    • DOI

      10.1016/j.anl.2011.11.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 内リンパ嚢におけるPannexin1の発現2013

    • Author(s)
      宮下武憲、森望
    • Organizer
      第23回日本耳科学会
    • Place of Presentation
      宮崎市
    • Year and Date
      2013-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] イソプロテレノールによる内リンパ圧に対する影響2013

    • Author(s)
      稲本隆平、宮下武憲、森望
    • Organizer
      第23回日本耳科学会
    • Place of Presentation
      宮崎市
    • Year and Date
      2013-11-26
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] マウス内リンパ嚢上皮細胞におけるProx1の発現2013

    • Author(s)
      宮下武憲、James Burford , Young-Kwon, Hong, Janos Peti-Peterdi,森望
    • Organizer
      第31回耳鼻咽喉科ニューロサイエンス研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2013-08-24
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ラット内リンパ嚢におけるcystic fibrosis transmembrane conductance regulator (CFTR)の発現2013

    • Author(s)
      松原あい、宮下武憲、稲本隆平、星川広史、森望
    • Organizer
      第31回耳鼻咽喉科ニューロサイエンス研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2013-08-24
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] マウス内リンパ嚢におけるProx1の発現2013

    • Author(s)
      宮下武憲、森望
    • Organizer
      第114回日本耳鼻咽喉科学会
    • Place of Presentation
      札幌市
    • Year and Date
      2013-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] イソプロテレノールによる内リンパ圧に対する影響2013

    • Author(s)
      稲本隆平、宮下武憲、森望
    • Organizer
      第114回日本耳鼻咽喉科学会
    • Place of Presentation
      札幌市
    • Year and Date
      2013-05-18
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] マウス内リンパ嚢におけるProx1の発現2013

    • Author(s)
      宮下武憲、森  望
    • Organizer
      第114回日本耳鼻咽喉科学会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 内リンパ嚢におけるPannexin1の発現2013

    • Author(s)
      宮下武憲、森  望
    • Organizer
      第23回日本耳科学会
    • Place of Presentation
      宮崎市
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス内リンパ嚢におけるProx1の発現2013

    • Author(s)
      宮下 武憲、森 望
    • Organizer
      第114回日本耳鼻咽喉科学会総会・学術講演会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウス鼓膜におけるリンパ管-正常鼓膜での局在と鼓膜再生時の反応-2012

    • Author(s)
      宮下武憲、森望
    • Organizer
      日本耳鼻咽喉科学会第38回四国四県地方部会連合学会
    • Place of Presentation
      高知市
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス鼓膜におけるリンパ系-鼓膜再生時の反応-2012

    • Author(s)
      宮下武憲、森望
    • Organizer
      第22回日本耳科学会
    • Place of Presentation
      名古屋市
    • Year and Date
      2012-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 鼓膜のリンパ管局在、鼓膜再生時の反応2012

    • Author(s)
      宮下武憲、James Burford, Young-Kwon Hong、森望、Janos Peti-Peterdi
    • Organizer
      第30回耳鼻咽喉科ニューロサイエンス研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2012-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット内リンパ嚢におけるアドレナリンレセプターの発現2012

    • Author(s)
      松原あい、宮下武憲、稲本隆平、森望
    • Organizer
      第30回耳鼻咽喉科ニューロサイエンス研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2012-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] イソプロテレノールの内耳への影響2012

    • Author(s)
      稲本隆平、宮下武憲、森望
    • Organizer
      第30回耳鼻咽喉科ニューロサイエンス研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2012-08-25
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.kms.ac.jp/~jibika/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2012-11-27   Modified: 2019-07-29  

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