Investigation and clinical application of corneal target cell-induced culture

• Project/Area Number: 24890157
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Ehime University
• Principal Investigator: TOMOYUKI Inoue 愛媛大学, 医学(系)研究科(研究院), 講師 (50432539)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 角膜 / 角膜上皮 / 移植 / 再生医療 / 幹細胞 / 角膜移植

Research Abstract

We investigated the differing characteristics of putative limbal niche cells (LNCs) and limbal stromal cells (LSCs) in the maintenance of limbal epithelial stem cells in the cornea. The colony-forming efficiency of limbal epithelial progenitor cells and the epithelial cell sheets in LNCs group was significantly better than that in the LSCs group. We developed a genetically modified line of human dermal fibroblast cells, which maintained immortalization, visualization, and eliminable characteristics. All corneal epithelial cell sheets were well stratified.

Research Products

• [Journal Article] Differences between niche cells and limbal stromal cells in maintenance of corneal limbal stem cells (2014)
  • Author(s): Li Y, Inoue T, Takamatsu F, Kobayashi T, Shiraishi A, Maeda N, Ohashi Y, Nishida K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55(3) Pages: 1453-62
  • Peer Reviewed
• [Journal Article] Differences between niche cells and limbal stromal cells in maintenance of corneal limbal stem cells (2014)
  • Author(s): Li Y, Inoue T, Takamatsu F, Kobayashi T, Shiraishi A, Maeda N, Ohashi Y, Nishida K.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55 Issue: 3 Pages: 1453-62
  • DOI: 10.1167/iovs.13-13698
  • Peer Reviewed / Open Access
• [Journal Article] Development of genetically modified human dermal fibroblast feeder cells for ocular surface regeneration medicine (2013)
  • Author(s): Li Y, Inoue T, Takamatsu F, Maeda N, Ohashi Y, Nishida
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54 Pages: 7522-31
  • Peer Reviewed
• [Journal Article] Development of genetically modified human dermal fibroblast feeder cells for ocular surface regeneration medicine. (2013)
  • Author(s): Li Y, Inoue T, Takamatsu F, Maeda N, Ohashi Y, Nishida.
  • Journal Title: Investigative Ophthalmology Visual Science Volume: 54 Issue: 12 Pages: 7522-7531
  • DOI: 10.1167/iovs.13-12870
  • Peer Reviewed