| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
We started the analysis of the axonal projection from the spinal trigeminal nucleus caudalis (Sp5C) in rat brain by a single neuron labeling method with Sindbis viral vectors. First, we considered how to approach to the Sp5C anatomically. As a result, it became possible to inject into target coordinates with high reproducibility by exposing not bregma but obex to make standard coordinates. Next, we injected the high concentration Sindbis viral vectors into the Sp5C and examined whether it would be satisfactory using as an anterograde tracer. By the exploratory experiment, the protocol for visualizing a single Sp5C neuron by Sindbis viral vectors was nearly completed.
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