Mechanism of neurite outgrowth promoting effect of LOTUS, an antagonist for Nogo receptor

• Project/Area Number: 24890187
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Yokohama City University
• Principal Investigator: KURIHARA Yuji 横浜市立大学, 生命医科学研究科, 特任助教 (00634552)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 神経科学 / 突起伸長 / シグナル伝達 / 神経再生

Research Abstract

LOTUS functions as the antagonist for Nogo receptor-1 (NgR1) which restricts functional recovery after damages to the central nervous system. We found that LOTUS also promotes neurite outgrowth. In this study, I analyzed the promoting effect of LOTUS on neurite outgrowth. Using the neurons from ngr1-deficient mice, I investigated the molecule(s) which mediate the promoting effect of LOTUS on neurite outgrowth. I found that LOTUS interacts with the molecule(s) except for NgR1 and the unidentified LOTUS binding molecule(s) mediate the promoting action of LOTUS on neurite outgrowth.

Research Products

• [Journal Article] Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones (2013)
  • Author(s): Iketani M., Iizuka A., Sengoku K., Kurihara Y., Nakamura F., Sasaki Y., Sato Y., Yamane M., Matsushita M., Nairn A.C., Takamatsu K., Goshima Y., Takei K.
  • Journal Title: Dev. Neurosci Volume: 73 Issue: 3 Pages: 230-246
  • DOI: 10.1002/dneu.22058
  • Peer Reviewed
• [Journal Article] LOTUSによる神経回路形成機構 (2013)
  • Author(s): 栗原裕司,竹居光太郎
  • Journal Title: Annual Review神経2013 Volume: 2013年発行巻 Pages: 63-67
  • URL: http://www.chugaiigaku.jp/item/detail.php?id=1180
• [Journal Article] LOTUSによる神経回路形成機構 (2013)
  • Author(s): 栗原裕司
  • Journal Title: Annual Review 神経 2013 Volume: 2013年発行巻
• [Presentation] LOTUSの神経突起伸長作用 (2013)
  • Author(s): 栗原裕司,伊藤拓夢,池谷真澄,五嶋良郎,竹居光太郎
  • Organizer: 第36回日本神経科学大会&第56回日本神経化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-21
• [Presentation] LOTUS functions as a potent antagonist for Nogo receptor (2012)
  • Author(s): Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry & The 55th Annual Meeting of the Japanese Society for Neurochemistry Kobe
  • Place of Presentation: Kobe
  • Year and Date: 2012-10-01
• [Presentation] Nogo受容体に対するLOTUSの拮抗作用 (2012)
  • Author(s): 栗原裕司,池谷真澄,伊藤拓夢,西山邦幸,榊原裕介,五嶋良郎,竹居光太郎
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] LOTUSの神経突起伸長作用
  • Author(s): 栗原 裕司，伊藤 拓夢，池谷 真澄，五嶋 良郎，竹居 光太郎
  • Organizer: 第36回日本神経科学大会・第56回日本神経化学会大会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] Nogo受容体に対するLOTUSの拮抗作用
  • Author(s): 栗原 裕司
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] LOTUS functions as a potent antagonist for Nogo receptor
  • Author(s): 栗原 裕司
  • Organizer: 第11回アジア太平洋神経化学会大会・第55回日本神経化学会大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県）