Development of the novel biomarker for tissue damage using polyamines and their metabolites

• Project/Area Number: 24890195
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: UEMURA Takeshi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50401005)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ポリアミン / アクロレイン / 肝臓 / アルコール / アセトアルデヒド / スペルミン酸化酵素 / エタノール / バイオマーカー

Research Abstract

The objective of this research is to develop a novel test for the identification of the cause of death in legal autopsy cases. We found that alcohol consumption induced spermine oxidase (SMO) at the transcriptional level in the liver and generated acrolein by polyamine metabolism to exert cytotoxicity. Our results indicate that polyamines and acrolein levels in liver tissues can be used as a novel biomarker for tissue damage caused by alcohol consumption.

Research Products

• [Journal Article] Acetaldehyde-induced cytotoxicity involves induction of spermine oxidase at the transcriptional level (2013)
  • Author(s): Uemura T., Tanaka Y., Higashi K., Miyamori D., Takasaka T., Nagano T., Toida T., Yoshimoto K., Igarashi K., and Ikegaya H
  • Journal Title: Toxicology Volume: 310C Pages: 1-7
• [Journal Article] Acetaldehyde-induced cytotoxicity involves induction of spermine oxidase at the transcriptional level. (2013)
  • Author(s): Uemura, T. et al.
  • Journal Title: Toxicology Volume: 310 Pages: 1-7
  • DOI: 10.1016/j.tox.2013.05.008
  • Peer Reviewed
• [Presentation] Acetaldehyde-induced cytoxicity involves induction of spermine oxidase at the transcrptional level (2013)
  • Author(s): Uemura T, Tanaka Y, Higashi K, Miyamori D, Takasaka T, Nagano T, Toida T, Yoshimoto K, Igarashi K, and Ikegaya H
  • Organizer: Gordon Research Conference on Polyamines 2013
• [Presentation] アセトアルデヒドの肝細胞毒性には，スペルミンオキシダーゼが関与する (2013)
  • Author(s): 植村武史，高坂友和，宮森大輔，吉本寛司，池谷 博
  • Organizer: 第97次日本法医学会学術全国集会
  • Place of Presentation: 札幌
• [Presentation] Acetaldehyde-induced ccytoxicity involves induction of spermine oxidase at the transcrptional level (2013)
  • Author(s): Uemura T*, Tanaka Y, Higashi K, Miyamori D, Takasaka T, Nagano T, Toida T, Yoshimoto K, Igarashi K, and Ikegaya H
  • Organizer: Gordon Research Conference on Polyamines 2013
  • Place of Presentation: New Hampshire, USA
• [Presentation] Regulation of cellular polyamines by transport, protein sorting and metabolism (2013)
  • Author(s): Uemura T, Stringer DE, Blohm-Mangone KA, Ikegaya H, Gerner EW
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] アセトアルデヒドは、スペルミンオキシダーゼを転写レベルで誘導する (2013)
  • Author(s): 植村 武史
  • Organizer: 日本ポリアミン学会第4回大会
  • Place of Presentation: 仙台
• [Presentation] アセトアルデヒドは,スペルミンオキシダーゼを転写レベルで誘導する
  • Author(s): 植村武史,東恭平,戸井田敏彦,五十嵐一衛,池谷博
  • Organizer: 日本ポリアミン学会第4回年会
• [Presentation] Regulation of cellular polyamine levels by transport, protein sorting and metabolism
  • Author(s): Uemura T., Stringer D.E., Blohm-Mangone K., Gerner E.W
  • Organizer: 第86回日本生化学大会
• [Presentation] アセトアルデヒドの肝細胞毒性には,スペルミンオキシダーゼが関与する
  • Author(s): 植村武史,高坂友和,宮森大輔,吉本寛司,池谷博
  • Organizer: 第97次日本法医学会学術全国集会
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/hoi/
• [Remarks] 京都府立医科大学法医学教室
  • URL: http://www.f.kpu-m.ac.jp/k/hoi/
• [Remarks] 京都府立医科大学 法医学教室
  • URL: http://www.f.kpu-m.ac.jp/k/hoi/