| Project/Area Number |
24890201
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YAMAMOTO Kenta 京都府立医科大学, 医学研究科, 助教 (00636160)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生医療 / 骨芽細胞 / ダイレクト・リプログラミング |
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| Research Abstract |
Alveolar bone resorption associated with advanced periodontitis causes tooth loss and reduces quality of life (QOL). Transplantation of autologous osteoblasts into periodontal tissue may facilitate alveolar bone regeneration as a new cell therapy. In this study, we attempted to directly reprogram human somatic cells into osteoblast-like cells by transducing some transcription factor genes. ALP positive cells were induced from somatic cells at a high efficiency, and the resultant cells expressed osteoblast-specific factors and produced mineralized bone matrix. Moreover, the resultant cells displayed a similar gene expression profile as normal human osteoblasts. These findings suggest successful reprogramming of somatic cells directly into osteoblasts, which may provide new regenerative medicine for periodontal diseases.
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