• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Integrated Genetic Analysis of Allelic Imbalance and FGFR3 Mutation by SNP-based Pyrosequencing in Urothelial Cancer

Research Project

Project/Area Number 24890206
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNara Medical University

Principal Investigator

LUO Yi  奈良県立医科大学, 分子病理学, 助教 (30633797)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords尿路上皮がん / 診断 / 尿中膀胱癌診断 / 遺伝子変化
Research Abstract

In this study, we refer to the rapid and quantitative analytical methods in urine samples, a SNP-based pyrosequencing (PSQ) targeting regions of LOHs on 9p, 9q, 17p, and also exon 7, 10 which are hotspots of FGFR3 mutations. Material and Methods: A total of 7 markers were designed to amplify targeting SNP regions on 9p, 9q and 17p. And 2 markers were designed to validate FGFR3 sequence including 2 point mutated hotspot in each marker in exon 7 and 10. Results: A total of 116 UC samples are analyzed. In the tissue analysis, 85.0% of UC showed either LOH or FGFR3 mutation. In the analysis of urine sediments, 75.0% of urine obtained from UC patients’ showed genetic alterations. Urine cytology could detect only 44.0% of UCs in the same cohort. No genetic alterations were detected in 20 healthy urine sediments. Conclusions: PSQ were the feasible assay for detect genetic alterations such as LOH and point mutation from small cancer cell populations.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )

URL: 

Published: 2012-11-27   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi