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The role of Src-p130Cas axis in osteoclastic bone resorption

Research Project

Project/Area Number 24890214
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionSaitama Medical University (2013)
Kyushu Dental College (2012)

Principal Investigator

OSAWA Kenji  埼玉医科大学, 医学部, 助教 (70638238)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords骨代謝 / 骨吸収 / 破骨細胞 / 細胞内情報伝達 / p130Cas / c-Src / Integrin / p130 Cas
Research Abstract

Osteoclast-specific p130Cas conditional knockout mice (p130Cas cKO) exhibit a high bone mass phenotype caused by defect in multinucleation and cytoskeleton organization causing bone resorption deficiency. Bone marrow cells from p130Cas cKO were able to differentiate into osteoclasts and wild-type cells in vitro. However, osteoclasts from p130Cas cKO failed to form actin rings and resorb pits on dentine slices. Although the initial events of osteoclast attachment were intact, the Rac1 activity that organizes the actin cytoskeleton was reduced, and its distribution was disrupted in p130Cas cKO osteoclasts. Dedicator of cytokinesis 5 (Dock5), a Rho family guanine nucleotide exchanger, failed to associate with Src or Pyk2 in osteoclasts in the absence of p130Cas. These results strongly indicate that p130Cas plays pivotal roles in osteoclastic bone resorption

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (20 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (17 results) (of which Invited: 2 results)

  • [Journal Article] RelB-induced Expression of Cot, an MAP3K Family Member, Rescues RANKL-induced Osteoclastogenesis in Alymphoplasia Mice by Promoting NF-κB2 Processing by IKKα.2014

    • Author(s)
      Taniguchi R, Fukushima H, Osawa K, Maruyama T, Yasuda H, Weih F, Doi T, Maki K, Jimi E.
    • Journal Title

      J Biol Chem.

      Volume: 289 Issue: 11 Pages: 7349-7361

    • DOI

      10.1074/jbc.m113.538314

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] p130Cas plays important roles in osteoclastic bone resorption2013

    • Author(s)
      Nagai Y, Osawa k, Fukushima H, Tamura Y, Aoki K, Ohya K, Yasuda H, Hikiji H, Kato S, Honda H, Nakamura I, Maki K, Jimi E
    • Journal Title

      J. Bone Miner. Res

      Volume: 28 Issue: 12 Pages: 2449-2462

    • DOI

      10.1002/jbmr.1936

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disruption of NF-κB1 prevents bone loss caused by mechanical unloading.2013

    • Author(s)
      Nakamura H, Aoki K, Masuda W, Alles N, Nagano K, Fukushima H, Osawa K, Yasuda H, Nakamura I, Mikuni-Takagaki Y, Ohya K, Maki K, Jimi E.
    • Journal Title

      J Bone Miner Res.

      Volume: 28 Issue: 6 Pages: 1457-1467

    • DOI

      10.1002/jbmr.1866

    • NAID

      10031169015

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] p130Casによる破骨細胞の骨吸収制御機構2013

    • Author(s)
      大澤賢次
    • Organizer
      第16回骨代謝研究会
    • Place of Presentation
      慶應大学(東京都新宿区)
    • Year and Date
      2013-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] p130Casの破骨細胞における骨吸収能発現のメカニズム2013

    • Author(s)
      大澤賢次、福島秀文、田村幸彦、青木和広、大谷啓一、牧憲司、自見英治郎
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 進行性骨化性線維異形成症から同定された2種類のALK2変異体はII型受容体に対する感受性が異なる2013

    • Author(s)
      藤本舞、大澤賢次、古株彰一郎、須田直人、片桐岳信
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規NF-κB阻害剤は口腔癌による顎骨浸潤を抑制する2013

    • Author(s)
      多田幸代、福島秀文、大澤賢次、自見英治郎
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子NF-κBによる骨形成調節機構について2013

    • Author(s)
      大澤賢次
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] p130CasはSrc/Pyk2/Dock5と会合し、Rac1の活性を制御することで破骨細胞の骨吸収を制御する2013

    • Author(s)
      大澤賢次、福島秀文、牧憲司、保田尚孝、田村幸彦、青木和広、大谷啓一、加藤茂明、仲村一郎、自見英治郎
    • Organizer
      第31回日本骨代謝学会学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2013-05-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] FOPの変異ALK2は異所性骨化を誘導する2013

    • Author(s)
      大澤賢次、宮本阿礼、塚本翔、水田誉人、片桐岳信
    • Organizer
      第11回RCGMフロンティアシンポジウム
    • Place of Presentation
      埼玉医科大学(埼玉県日高市)
    • Related Report
      2013 Final Research Report
  • [Presentation] FOPの変異ALK2は異所性骨化を誘導する。2013

    • Author(s)
      大澤賢次、宮本阿礼、塚本翔、藤本舞、水田誉人、片桐岳信
    • Organizer
      第11回RCGMフロンティアシンポジウム
    • Place of Presentation
      埼玉医科大学(埼玉県日高市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] p130Cas plays important roles in osteoclastic bone resorption2012

    • Author(s)
      Yoshie Nagai, Kenji Osawa, Hidefummi Fukushima, Kenshi Maki, Eijiro Jimi
    • Organizer
      The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology Future Technology in Medical & Dental Areas
    • Place of Presentation
      九州工業大学(福岡県北九州市)
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] p130Casは破骨細胞の機能発現に重要な役割をもつ2012

    • Author(s)
      永井香絵、福島秀文、大澤賢次、田村幸彦、青木和広、大谷啓一、中村仁美、牧憲司、自見英治郎
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      奥羽大学(福島県上郡市)
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] NF-κBのp100のプロセシングは骨代謝において重要である2012

    • Author(s)
      大澤賢次、福島秀文、Alles Neil、青木和広、張皿、大谷啓一、自見英治郎
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      奥羽大学(福島県上郡市)
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子 NF-κB による骨形成調節機構について

    • Author(s)
      大澤賢次
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 新規 NF-κB 選択的阻害剤は口腔癌による顎骨浸潤を抑制する

    • Author(s)
      多田 幸代、福島 秀文、大澤 賢次、自見 英治郎
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 進行性骨化性線維異形成症から同定された 2 種類の ALK2 変異体は II 型受容体に対する感受性が異なる

    • Author(s)
      藤本 舞、大澤 賢次、古株 彰一郎、須田 直人、片桐 岳信
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山国際会議場(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] p130Casによる破骨細胞の骨吸収制御機構

    • Author(s)
      大澤賢次
    • Organizer
      第16回骨代謝研究会
    • Place of Presentation
      慶応大学(東京都新宿区)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] P130Casは破骨細胞の機能発現に重要な役割をもつ

    • Author(s)
      永井香絵、福島秀文、大澤賢次、田村幸彦、青木和広、大谷啓一、中村仁美、牧憲司、自見英治郎
    • Organizer
      第54回 歯科基礎医学会学術大会
    • Place of Presentation
      奥羽大学(福島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] p130Cas plays important roles in osteoclastic bone resorption

    • Author(s)
      Yoshie Nagai, Kenji Osawa, Hidefumi Fukushima, Kenshi Maki, Eijiro Jimi
    • Organizer
      The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology Future Technology in Medical & Dental Areas
    • Place of Presentation
      九州工業大学(福岡県)
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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