Exploring a novel cardiomyocyte cell cycle regulator by utilizing a fluorescent ubiquitination-based cell cycle indicator

• Project/Area Number: 24890227
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Keio University
• Principal Investigator: HASHIMOTO Hisayuki 慶應義塾大学, 医学部, 助教 (90528390)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞周期 / ライブイメージング / 心臓発生 / 再生医療 / 心筋再生 / 心不全

Research Abstract

The minimal capacity of mature cardiomyocte regeneration is one of the factors which determines the poor prognosis of heart failure. If we can proliferate cardiomyocytes and regenerate the heart, we will be able to completely cure heart failure. We planned to explore a novel cardiomyocyte cell cycle regulator by utilizing a fluorescent ubiquitination-based cell cycle indicator. We identified a protein which the expression level changes during the proliferation phase of cell cycle. We are analyzing the function of this candidate protein by over-expressing and deleting it in cardiomyocyte.

Research Products

• [Journal Article] Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte. (2014)
  • Author(s): Hashimoto H, Yuasa S, Tabata H, Tohyama S, Hayashiji N, Hattori F, Muraoka N, Egashira T, Okata S, Yae K, Seki T, Nishiyama T, Nakajima K, Sakaue-Sawano A, Miyawaki A and Fukuda K.
  • Journal Title: J.Mol. Cell. Cardiol. Volume: S0022-2828(14) Pages: 97-2
  • DOI: 10.1016/j.yjmcc.2014.03.020
  • Peer Reviewed
• [Journal Article] Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte (2014)
  • Author(s): Hisayuki Hashimoto
  • Journal Title: Journal of Molecular and Cellular Cardiology Volume: 印刷中
  • Peer Reviewed
• [Presentation] A Novel Cardiac ex vivo Culture System Revealed the Elongation of Cell Cycle Phases in Cardiomyocytes During Development (2013)
  • Author(s): Hisayuki Hashimoto, Shinsuke Yuasa, Hidenori Tabata, Shugo Tohyama, Toru Egashira, Tomohisa Seki, Kazunori Nakajima, Keiichi Fukuda
  • Organizer: 第30回国際心臓研究学会日本部会総会
  • Place of Presentation: 米国・サンディエゴ
  • Year and Date: 2013-06-29
• [Presentation] A Novel Cardiac ex vivo Culture System Revealed the Elongation of Cell Cycle Phases in Cardiomyocytes During Development
  • Author(s): Hisayuki Hashimoto
  • Organizer: International Society for Heart Research (ISHR) The 30 th Annual Meeting of the International Society for Heart Research Japanese Section
  • Place of Presentation: San Diego, USA