| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
A quantitative analysis of Proractin induced protein(PIP) mRNA in junctional epithelium (JE) and oral gingival epithelium (OGE) of both conventional mice and germ-free mice was performed using laser microdissection and real-time polymerase chain reaction. Real-time PCR analysis indicated that PIP expressions were mainly detected in JE ofconventional mice not detected in OGE. Expression of PIP after LPS stimulation in cuitured human oral epithelial cells was higher expression most 24 hours after stimulation.
Further, the results of immunohistochemical analysis, expression of PIP was observed in JE of each of the conventional mice and germ-free mice, expression was not observed in the OGE,respectively.
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