Role of CTGF/CCN2 in reparative dentin formation

• Project/Area Number: 24890258
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: MUROMACHI Koichiro 日本大学, 松戸歯学部, 助手(専任扱) (50637072)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 修復象牙質 / CTGF/CCN2 / BMP-1 / 象牙質・歯髄複合体 / 歯髄/象牙質複合体

Research Abstract

The aim of this study is to elucidate the expression and role of connective tissue growth factor/CCN family 2 (CTGF/CCN2) in reparative dentin formation. Results indicated that specific signal for CTGF/CCN2 and BMP-1 were increased in the layer of odontoblast-like cells subjacent to the dental caries in human teeth. BMP-1 was capable of inducing the expression of CTGF/CCN2 independently of protease activity in primary culture of human dental pulp cells. Exogenously added fluorescence-labeled BMP-1 was internalized into human dental pulp cells. Furthermore, a potent dynamin inhibitor clearly suppressed BMP-1-induced CCN2/CTGF expression. These findings indicate that novel mechanism of CTGF/CCN2 expression in reparative dentinogenesis.

Research Products

• [Journal Article] Tumor necrosis factorαstimulates expression and secretion of urokinase plasminogen activator in human dental pulp cells (2012)
  • Author(s): Narita T, Muromachi K, Kamio N, Nakao S, Matsushima K, Hashizume H
  • Journal Title: Journal of Oral Science Volume: 54 Pages: 329-326
  • NAID: 130003318541
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor α stimulates expression and secretion of urokinase plasminogen activator in human dental pulp cells (2012)
  • Author(s): Takanori Narita, Koichiro Muromachi, Naoto Kamio, Sumi Nakao, Kiyoshi Matsushima, Hideki Hashizume
  • Journal Title: Journal of Oral Science Volume: 54 Issue: 4 Pages: 329-336
  • DOI: 10.2334/josnusd.54.329
  • NAID: 130003318541
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed
• [Presentation] ヒト象牙質・歯髄複合体におけるメタロプロテアーゼとCTGF/CCN2発現 (2013)
  • Author(s): 室町幸一郎,神尾直人,松島潔
  • Organizer: 第55回歯科基礎医学会学術大会サテライトシンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-20
  • Invited
• [Presentation] PlasminによるCOX-2産生シグナルと細胞内カルシウムイオン濃度の変化 (2013)
  • Author(s): 神尾直人,室町幸一郎,葉山朋美,松島潔
  • Organizer: 日本歯科保存学会2013年度秋季学術大会(第139回)
  • Place of Presentation: 秋田
• [Presentation] BMP-1 promotes CTGF/CCN2 expression independently of protease activity in human carious teeth (2013)
  • Author(s): Muromachi K, Kamio N, Matsushima K
  • Organizer: IFEA The 9th World Endodontic Congress
  • Place of Presentation: Tokyo, JAPAN
• [Presentation] BMP-1 promotes CTGF/CCN2 expression independently of protease activity in human carious teeth (2013)
  • Author(s): Koichiro MUROMACHI, Naoto KAMIO, Kiyoshi MATSUSHIMA
  • Organizer: The 9th World Endodontic Congress
  • Place of Presentation: Tokyo International Forum (Tokyo)
• [Presentation] PlasminによるCOX-2産生シグナルと細胞内カルシウムイオン濃度の変化 (2013)
  • Author(s): 神尾 直人, 室町 幸一郎, 葉山朋美, 松島 潔
  • Organizer: 日本歯科保存学会 2013年度秋季学術大会（第139回）
  • Place of Presentation: 秋田県総合生活文化会館（アトリオン）（秋田県）
• [Presentation] BMP-1 promotes CTGF/CCN2 expression independently of protease activity in human carious teeth (2013)
  • Author(s): 室町 幸一郎
  • Organizer: The 9th World Endodontic Congress
  • Place of Presentation: 東京国際フォーラム（東京）
• [Presentation] ヒト象牙質･歯髄複合体におけるメタロプロテアーゼとCTGF/CCN2発現
  • Author(s): 室町 幸一郎, 神尾 直人, 松島 潔
  • Organizer: 第５５回 歯科基礎医学会学術大会（サテライトシンポジウム）
  • Place of Presentation: 岡山コンベンションセンター（岡山県）
  • Invited