| Project/Area Number |
24890263
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
|
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| Research Institution | Osaka University (2013)
Musashino University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 構造生物学 / HMGB / IL-1 / 分子間相互作用 |
|---|
| Research Abstract |
Basically HMGB1 plays a role in gene transcription in nucleus. However, leaked HMGB1 out of the cells induce inflammation around the cell. The inflammatory reaction can be augmented by interacting with IL-1beta, which is one of the potent inflammatory cytokines.
Recombinant HMGB1 and IL-1beta proteins could be sucsessfully over-expressed by using Escherichia coli expression system. Then, we could highly purified the recombinant HMGB1 and IL-1beta proteins by performing several types of chromatographic experiments. Furthermore, binding affinity between the recombinant HMGB1 and IL-1beta proteins were investigated by measuring fluorescence depolarization.
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