| Project/Area Number |
24890270
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
HOSHI Masato 鈴鹿医療科学大学, 保健衛生学部, 助教 (40633996)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Kuniaki 京都大学, 医学研究科, 教授 (80262765)
SEISHIMA Mitsuru 岐阜大学, 医学系研究科, 教授 (10171315)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | インドールアミン酸素添加酵素 / トリプトファン代謝 / α-ガラクトシルセラミド / NKT細胞免疫療法 / 腫瘍 / IDO / α- ガラクトシルセラミド |
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| Research Abstract |
The role of indoleamine 2,3-dioxygenase (Ido) in the L-tryptophan (Trp)-kynurenine (Kyn) pathway after lung metastasis model by injecting B16F10 cells was investigated. We used mice treated with 1-methyl-D or L-tryptophan (D or L-1MT), an inhibitor of Ido1 or Ido2, to study the importance of Trp-Kyn pathway metabolites. On day 7 after B16F10 cells, we administrated each NKT activated ligands (alpha-GalCer and 7DW8-5). The levels of serum IFN-g and Ido activity in mice 6 hours after alpha-GalCer injection were higher than those in non-treated mice. Furthermore, the levels of Ido1 and Ido2 mRNA and protein expression in the lung and serum Trp metabolites from mice treated with alpha-GalCer were significantly higher than those from non-treated mice. Moreover, the anti-tumor effect in the lung from mice treated with alpha-GalCer and D- or L-1MT combined administration was markedly improved compared to that in mice treated with -GalCer single administration.
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