The investigation of immune regulation by IDO in NKT cell-mediated immunotherapy
Project/Area Number |
24890270
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Laboratory medicine
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Research Institution | Suzuka University of Medical Science |
Principal Investigator |
HOSHI Masato 鈴鹿医療科学大学, 保健衛生学部, 助教 (40633996)
|
Co-Investigator(Renkei-kenkyūsha) |
SAITO Kuniaki 京都大学, 医学研究科, 教授 (80262765)
SEISHIMA Mitsuru 岐阜大学, 医学系研究科, 教授 (10171315)
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Project Period (FY) |
2012-08-31 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | インドールアミン酸素添加酵素 / トリプトファン代謝 / α-ガラクトシルセラミド / NKT細胞免疫療法 / 腫瘍 / IDO / α- ガラクトシルセラミド |
Research Abstract |
The role of indoleamine 2,3-dioxygenase (Ido) in the L-tryptophan (Trp)-kynurenine (Kyn) pathway after lung metastasis model by injecting B16F10 cells was investigated. We used mice treated with 1-methyl-D or L-tryptophan (D or L-1MT), an inhibitor of Ido1 or Ido2, to study the importance of Trp-Kyn pathway metabolites. On day 7 after B16F10 cells, we administrated each NKT activated ligands (alpha-GalCer and 7DW8-5). The levels of serum IFN-g and Ido activity in mice 6 hours after alpha-GalCer injection were higher than those in non-treated mice. Furthermore, the levels of Ido1 and Ido2 mRNA and protein expression in the lung and serum Trp metabolites from mice treated with alpha-GalCer were significantly higher than those from non-treated mice. Moreover, the anti-tumor effect in the lung from mice treated with alpha-GalCer and D- or L-1MT combined administration was markedly improved compared to that in mice treated with -GalCer single administration.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Kynurenine production mediated by indoleamine 2,3-dioxygenase aggravates liver injury in HBV-specific CTL-induced fulminant hepatitis2014
Author(s)
Ohtaki H, Ito H, Ando K, Ishikawa T, Hoshi M, Ando T, Takamatsu M, Hara A, Moriwaki H, Saito K, Seishima M
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Journal Title
Biochim Biophys Acta
Volume: S0925-4439(14)
Pages: 102-1
Related Report
Peer Reviewed
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[Journal Article] Indoleamine 2,3-dioxygenase 1 is upregulated in activated microglia in mice cerebellum during acute viral encephalitis2014
Author(s)
Taguchi A, Niwa M, Hoshi M, Saito K, Masutani T, Hisamatsu K, Kobayashi K, Hatano Y, Tomita H, Hara A
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Journal Title
Neurosci Lett
Volume: 3564
Pages: 120-5
DOI
Related Report
Peer Reviewed
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[Journal Article] Indoleamine 2,3-dioxygenase 1 is upregulated in activated microglia in mice cerebellum during acute viral encephalitis.2014
Author(s)
Taguchi A, Niwa M, Hoshi M, Saito K, Masutani T, Hisamatsu K, Kobayashi K, Hatano Y, Tomita H, Hara A.
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Journal Title
Neurosci Lett
Volume: 564
Pages: 120-5
Related Report
Peer Reviewed
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[Journal Article] IDO1 plays an immunosuppressive role in 2, 4, 6-trinitrobenzene sulfate-induced colitis in mice2013
Author(s)
Takamatsu M, Hirata A, Ohtaki H, Hoshi M, Hatano Y, Tomita H, Kuno T, Saito K, Hara A
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Journal Title
J Immunol
Volume: 15;191(6)
Issue: 6
Pages: 3057-64
DOI
Related Report
Peer Reviewed
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[Journal Article] IDO1 plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice.2013
Author(s)
Takamatsu M, Hirata A, Ohtaki H, Hoshi M, Hatano Y, Tomita H, Kuno T, Saito K, Hara A.
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Journal Title
J Immunol
Volume: 191(6)
Pages: 3057-64
Related Report
Peer Reviewed
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[Journal Article] -Epigallocatechin gallate inhibits the expression of indoleamine 2,3-dioxygenase in human colorectal cancer cells2012
Author(s)
Ogawa K, Hara T, Shimizu M, Nagano J, Ohno T, Hoshi M, Ito H, Tsurumi H, Saito K, Seishima M, Moriwaki H
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Journal Title
Oncol Lett
Volume: 4(3)
Pages: 546-550
URL
Related Report
Peer Reviewed
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[Journal Article] Inhibition of increased indoleamine 2, 3-dioxygenase activity attenuates Toxoplasma gondii replication in the lung during acute infection2012
Author(s)
Murakami Y, Hoshi M, Hara A, Takemura M, Arioka Y, Yamamoto Y, Matsunami H, Funato T, Seishima M, Saito K
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Journal Title
Cytokine
Volume: 59(2)
Issue: 2
Pages: 245-51
DOI
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Peer Reviewed
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[Journal Article] Suppression of azoxymethane-induced colonic preneoplastic lesions in rats by 1-methyltryptophan, an inhibitor of indoleamine 2, 3-dioxygenase2012
Author(s)
Ogawa K, Hara T, Shimizu M, Ninomiya S, Nagano J, Sakai H, Hoshi M, Ito H, Tsurumi H, Saito K, Seishima M, Tanaka T, Moriwaki H
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Journal Title
Cancer Sci
Volume: 103
Issue: 5
Pages: 951-958
DOI
Related Report
Peer Reviewed
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