Nobel roles of sPLA2 that resolves inflammatory immune response

• Project/Area Number: 24890296
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: MIKI Yoshimi 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 研究員 (00632499)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脂質メディエーター / 抗炎症 / 自己免疫疾患 / 生体分子 / モデルマウス / ホスホリパーゼ / 皮膚炎 / ホスホリパーゼA2 / 脂質 / 皮膚疾患 / 樹状細胞 / DHA / レゾルビン / 脂肪酸

Research Abstract

The molecular entity of PLA2 subtypes acting upstream of antiinflammatory lipid mediators remains unknown. We show that secreted PLA2 group IID (PLA2G2D) is preferentially expressed in CD11c+ dendritic cells and macrophages and contributes to mobilization of a pool of polyunsaturated fatty acids that could serve as precursors for antiinflammatory/pro-resolving lipid mediators such as resolvin D1 and 15-deoxy-PGJ2. Our results highlight PLA2G2D as a "resolving sPLA2" and point to a potential use of this enzyme for treatment of inflammatory disorders.

Research Products

• [Journal Article] Lymphoid tissue phospholipase A_2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K., Taketomi, Y., Sato, H., Shimo, K., Kobayashi, T., Ishikawa, Y., Ishii, T., Nakanishi, H., Ikeda, K., Taguchi, R., Kabashima, K., Arita, M., Arai, H., Lambeau, G., Bollinger, J.M., Hara, S., Gelb, M.H., and Murakami, M
  • Journal Title: J. Exp. Med Volume: 210 Pages: 1217-1234
  • Peer Reviewed
• [Journal Article] 疾病における脂質代謝ネットワークの機能 (2013)
  • Author(s): 三木寿美,山本圭,村上誠
  • Journal Title: オレオサイエンス Volume: 13 Pages: 485-492
  • NAID: 130005122088
  • Peer Reviewed
• [Journal Article] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators. (2013)
  • Author(s): Miki Y, Yamamoto K, Taketomi Y, Sato H, Shimo K, Kobayashi T, Ishikawa Y, Ishii T, Nakanishi H, Ikeda K, Taguchi R, Kabashima K, Arita M, Arai H, Lambeau G, Bollinger JM, Hara S, Gelb MH, Murakami M.
  • Journal Title: J Exp Med. Volume: 210 Issue: 6 Pages: 1217-1234
  • DOI: 10.1084/jem.20121887
  • Peer Reviewed
• [Presentation] Group IID phospholipase A_2 resolves contact hypersensitivity and psoriasis by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K., Taketomi, Y., Gelb, M.H., Lambeau, G. and Murakami, M
  • Organizer: 13^<th> International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico
• [Presentation] H., Lambeau, G. and Murakami, M.: Epidermal phospholipase A_2 group IIF regulates keratinocyte differentiation and skin barrier through driving a unique set of lipid products (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M
  • Organizer: 13^<th> International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico
• [Presentation] IID型分泌性ホスホリパーゼA_2は抗炎症性脂質メディエーターを動員して皮膚炎を寛解する (2013)
  • Author(s): 三木寿美,山本圭,村上誠
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] IIF型分泌性ホスホリパーゼA_2は皮膚疾患の進行に寄与する (2013)
  • Author(s): 山本圭,三木寿美,佐藤真利子,椛島健治,石川由起雄,石井壽晴,村上誠
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Lymphoid tissue phospholipase A_2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K. and Murakami, M
  • Organizer: FASEB Science Reserch Conferences, Lysophospholipid and Other Related Mediators - From Bench to Clinic
  • Place of Presentation: Niseko, Japan
• [Presentation] Emerging roles of secreted phospholipase A_2s (2013)
  • Author(s): Murakami, M., Taketomi, Y., Miki, Y., Sato, H. and Yamamoto, K
  • Organizer: FASEB Science Reserch Conferences, Lysophospholipid and Other Related Mediators - From Bench to Clinic
  • Place of Presentation: Niseko, Japan
• [Presentation] IIF型分泌性ホスホリパーゼA_2は表皮の恒常性維持および病態に寄与する (2013)
  • Author(s): 山本圭,三木寿美,佐藤真利子,武富芳隆,池田和貴,中西広樹,田口良,村上誠
  • Organizer: 第55回日本脂質生化学会
  • Place of Presentation: 宮城県松島
• [Presentation] Group IIF phospholipase A_2 maintains skin homeastasis (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M. H., Lambeau, G. Murakami, M
  • Organizer: 5^<th> International Conference on Phospholipase A_2 Mediates Signaling in Translational Medicine
  • Place of Presentation: New Orleans, USA
• [Presentation] Group IID phospholipase A2 resolves contact hypersensitivity and psoriasis by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K., Taketomi, Y., Gelb, M. H., Lambeau, G., and Murakami, M.
  • Organizer: 13th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico
• [Presentation] IID型分泌性ホスホリパーゼA2は抗炎症性脂質メディエーターを動員して皮膚炎を寛解する (2013)
  • Author(s): 三木 寿美、山本 圭、村上 誠
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜、日本
• [Presentation] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K., and Murakami, M.
  • Organizer: FASEB Science Reserch Conferences, Lysophospholipid and Other Related Mediators - From Bench to Clinic
  • Place of Presentation: Niseko, Japan
• [Presentation] リン脂質代謝酵素IID型分泌性ホスホリパーゼA_2 (sPLA_2-IID)は炎症の寛解を制御する (2012)
  • Author(s): 三木寿美
  • Organizer: 平成24年度東京都医学総合研究所ポスター発表会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-03
• [Presentation] IID型分泌性ホスホリパーゼA_2の欠損は接触性皮膚炎の感作相後期においてリンパ節の炎症を増悪する (2012)
  • Author(s): 三木寿美,山本圭,村上誠
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 皮膚病態生理機能におけるIIE型分泌性ホスホリパーゼA_2の役割 (2012)
  • Author(s): 山本圭,佐藤弘泰,佐藤真利子,三木寿美,武富芳隆,村上誠
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] IID型分泌性ホスホリパーゼA2の欠損は接触性皮膚炎の感作相後期においてリンパ節の炎症を増悪する (2012)
  • Author(s): 三木寿美
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 皮膚病態生理機能におけるIIE型分泌性ホスホリパーゼA2の役割 (2012)
  • Author(s): 山本圭
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] IIF型分泌性ホスホリパーゼA2の欠損は皮膚癌形成を抑制する (2012)
  • Author(s): 佐藤真利子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] メタボリックシンドロームにおけるIID型、IIE型分泌性ホスホリパーゼA2の役割 (2012)
  • Author(s): 牛田絢子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://www.igakuken.or.jp/lipid/
• [Remarks] 雑誌論文(1)プレス発表:東京都医学研など、脂質成分が炎症にブレーキをかける仕組みを解明.マイナビニュース, 2013.5.27
  • URL: http://news.mynavi.jp/news/2013/05/27/112/
• [Remarks] 東京都医学総合研究所脂質代謝プロジェクト（村上研究室）
  • URL: http://www.igakuken.or.jp/lipid/
• [Remarks] 東京都医学総合研究所脂質代謝プロジェクト（村上研究室）
  • URL: http://www.igakuken.or.jp/lipid/