| Project/Area Number |
24890298
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2013)
National Research Institute for Child Health and Development (2012) |
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Principal Investigator |
SASAKI Norihiko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80639063)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 血管内皮細胞 / 加齢 / 老化 / 糖鎖 / iPS細胞 / 自然細胞老化 / ストレス性老化 / ヒトiPS細胞 / 分化誘導 |
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| Research Abstract |
To evaluate aging and senescence of human blood vessel in vitro, I have established human vascular endothelial cells, main component of blood vessel, from human induced pluripotent stem cells (iPS cells), which are various in age. The efficiency of endothelial differentiation is various and it has been suggested that various efficiency may be related to derivation age of iPS cells and expression pattern of glycoconjugates. Next, to evaluate aging and senescence of vascular endothelial cells derived from iPS cells, I tested assay of aging, replicative senescence and stress-induced senescence using primary human vascular endothelial cells. In primary human vascular endothelial cells, aging, replicative senescence and stress-induced senescence have been observed reproducibly.
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