The study of aldosterone production machinery by angiotensin II metabolite

• Project/Area Number: 24890308
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Hiroshima University (2013); Department of Clinical Research, National Hospital Organization Kure Medical Center (2012)
• Principal Investigator: OKI Kenji 広島大学, 大学病院, 病院助教 (30638995)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アルドステロン / アンギオテンシンII / 高血圧 / 副腎皮質

Research Abstract

Aldosterone which regulates blood pressure is produced by angiotensin II (A-II) in zona glomerulosa of adrenal cortex. We previously demonstrated that the metabolite of A-II negatively regulated A-II mediated aldosterone production in human adrenocortical cancer cell line. In this study, we clarified that the metabolite was angiotensin 1-7 and it inhibit aldosterone production after A-II stimulation. The function of angiotensin 1-7 on aldosterone production was mediated by MAS receptor. We showed a new aldosterone production machinery via MAS receptor and our results might be useful for new target of anti-hypertensive agents.

Research Products

• [Journal Article] Minireview : potassium channels and aldosterone dysregulation : is primary aldosteronism a potassium channelopathy? (2014)
  • Author(s): Gomez-Sanchez CE, Oki K
  • Journal Title: Endocrinology Volume: 155 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Angiotensin II and III metabolism and effects on steroid production in the HAC15 Human Adrenocortical Cell line (2013)
  • Author(s): Oki K
  • Journal Title: Endocrinology Volume: 154 Pages: 214-21
  • Peer Reviewed
• [Journal Article] Significantly high level of late-night free cortisol to creatinine ratio in urine specimen in patients with subclinical Cushing's syndrome (2013)
  • Author(s): Shiwa T, Oki K
  • Journal Title: Clin Endocrinol (Oxf) Volume: 79 Pages: 617-22
  • Peer Reviewed
• [Journal Article] Minireview: potassium channels and aldosterone dysregulation: is primary aldosteronism a potassium channelopathy? (2013)
  • Author(s): Gomez-Sanchez CE, Oki K.
  • Journal Title: Endocrinology Volume: 155 Issue: 1 Pages: 47-55
  • DOI: 10.1210/en.2013-1733
  • Peer Reviewed
• [Journal Article] Influence of adrenal subclinical hypercortisolism on hypertension in patients with adrenal incidentaloma (2012)
  • Author(s): Oki K
  • Journal Title: Exp Clin Endocrinol Diabetes Volume: 120 Pages: 244-7
  • Peer Reviewed
• [Journal Article] Potassium Channel Mutant KCNJ5 T158A Expression in HAC-15 Cells Increases Aldosterone Synthesis (2012)
  • Author(s): Oki K
  • Journal Title: Endocrinology Volume: 153 Pages: 1774-82
  • Peer Reviewed
• [Journal Article] The potassium channel Kir3.4 participates in angiotensin II-stimulated aldosterone production by a human adrenocortical cell line (2012)
  • Author(s): Oki K
  • Journal Title: Endocrinology Volume: 153 Pages: 4228-35
  • Peer Reviewed
• [Journal Article] Potassium channel mutant KCNJ5 T158A expression in HAC-15 cells increases aldosterone synthesis. (2012)
  • Author(s): Oki K, et al.
  • Journal Title: Endocrinology Volume: 153 Issue: 4 Pages: 1774-1782
  • DOI: 10.1210/en.2011-1733
  • Peer Reviewed
• [Journal Article] The potassium channel, Kir3.4 participates in angiotensin II-stimulated aldosterone production by a human adrenocortical cell line. (2012)
  • Author(s): Oki K, et al.
  • Journal Title: Endocrinology Volume: 153 Issue: 9 Pages: 4328-4335
  • DOI: 10.1210/en.2012-1241
  • Peer Reviewed
• [Journal Article] Influence of adrenal subclinical hypercortisolism on hypertension in patients with adrenal incidentaloma. (2012)
  • Author(s): Oki K, et al.
  • Journal Title: Exp Clin Endocrinol Diabetes Volume: 120 Issue: 04 Pages: 244-247
  • DOI: 10.1055/s-0032-1301896
  • Peer Reviewed
• [Journal Article] Angiotensin II and III metabolism and effects on steroid production in the HAC15 human adrenocortical cell line. (2012)
  • Author(s): Kenji Oki
  • Journal Title: Endocrinology Volume: 154(1): Issue: 1 Pages: 214-21
  • DOI: 10.1210/en.2012-1557
  • Peer Reviewed
• [Presentation] アルドステロン分泌の分子機構 (2013)
  • Author(s): 沖健司
  • Organizer: 第86日本内分泌学会総会
  • Place of Presentation: 金沢市
• [Presentation] miR-21 suppression attenuate cell proliferation and decrease aldosterone production via StAR repression in human adrenocortical cells (2013)
  • Author(s): Oki K
  • Organizer: International Symposium of Aldosterone and Related Substances in Hypertension
  • Place of Presentation: Sendai, Japan
• [Presentation] アルドステロン分泌の分子機構 (2013)
  • Author(s): 沖 健司
  • Organizer: 日本内分泌学会総会
  • Place of Presentation: 宮城県仙台市
  • Invited
• [Presentation] miR-21 suppression attenuate cell proliferation and decrease aldosterone production via StAR repression in human adrenocortical cells (2013)
  • Author(s): Kenji Oki, Maria W Plonczynski, Elise P Gomez-Sanchez, Celso E Gomez-Sanchez
  • Organizer: International Symposium of Aldosterone and Related Substances in Hypertension
  • Place of Presentation: Sendai
• [Presentation] Kir3.4(KCNJ5)によるアルドステロン分泌調節機構 (2012)
  • Author(s): 沖健司
  • Organizer: 第20回日本ステロイドホルモン学会
  • Place of Presentation: 仙台市
  • Year and Date: 2012-11-18
• [Presentation] Kir3.4(KCNJ5)によるアルドステロン分泌調節機構
  • Author(s): 沖健司，他
  • Organizer: 第20回日本ステロイドホルモン学会
  • Place of Presentation: 金沢市