Reconstituting human axial development in vitro

• Project/Area Number: 24H00569
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Kyoto University
• Principal Investigator: ALEV CANTAS 京都大学, 高等研究院, 教授 (30726477)
• Project Period (FY): 2024-04-01 – 2029-03-31
• Project Status: Granted (Fiscal Year 2025)
• Budget Amount: ¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000); Fiscal Year 2025: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2024: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: embryo model / primate development / neurulation / somitogenesis / notochord / ヒト胚発⽣ / 体外胚モデル / オルガノイド

Outline of Research at the Start

Building & extending upon our recent in vitro 3D model of human somitogenesis (axioloid) we propose to reconstitute and analyze human and non-human primate (monkey & ape) axial development in vitro. Our established axioloid v2.0 model with somites & neural tube will be extensively analyzed at the molecular & functional level, with a focus on the identification & validation of human & non-human primate-specific features associated with axial development & morphogenesis. Successful implementation of our research will enhance our still limited understanding of human & non-human primate biology.